Journal article
Anaplasma marginale Major Surface Protein 2 CD4+-T-Cell Epitopes Are Evenly Distributed in Conserved and Hypervariable Regions (HVR), Whereas Linear B-Cell Epitopes Are Predominantly Located in the HVR
Infection and immunity, Vol.72(12), pp.7360-7366
12/2004
Handle:
https://hdl.handle.net/2376/115740
PMCID: PMC529150
PMID: 15557669
Abstract
Organisms in the genus
Anaplasma
express an immunodominant major surface protein 2 (MSP2), composed of a central hypervariable region (HVR) flanked by highly conserved regions. Throughout
Anaplasma marginale
infection, recombination results in the sequential appearance of novel MSP2 variants and subsequent control of rickettsemia by the immune response, leading to persistent infection. To determine whether immune evasion and selection for variant organisms is associated with a predominant response against HVR epitopes, T-cell and linear B-cell epitopes were localized by measuring peripheral blood gamma interferon-secreting cells, proliferation, and antibody binding to 27 overlapping peptides spanning MSP2 in 16 cattle. Similar numbers of MSP2-specific CD4
+
T-cell epitopes eliciting responses of similar magnitude were found in conserved and hypervariable regions. T-cell epitope clusters recognized by the majority of animals were identified in the HVR (amino acids [aa] 171 to 229) and conserved regions (aa 101 to 170 and 272 to 361). In contrast, linear B-cell epitopes were concentrated in the HVR, residing within hydrophilic sequences. The pattern of recognition of epitope clusters by T cells and of HVR epitopes by B cells is consistent with the influence of protein structure on epitope recognition.
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Details
- Title
- Anaplasma marginale Major Surface Protein 2 CD4+-T-Cell Epitopes Are Evenly Distributed in Conserved and Hypervariable Regions (HVR), Whereas Linear B-Cell Epitopes Are Predominantly Located in the HVR
- Creators
- Jeffrey R Abbott - Program in Vector-borne Disease, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WashingtonGuy H Palmer - Program in Vector-borne Disease, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WashingtonChris J Howard - Program in Vector-borne Disease, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WashingtonJayne C Hope - Program in Vector-borne Disease, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WashingtonWendy C Brown - Program in Vector-borne Disease, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington
- Publication Details
- Infection and immunity, Vol.72(12), pp.7360-7366
- Academic Unit
- Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
- Publisher
- American Society for Microbiology
- Identifiers
- 99900547339001842
- Language
- English
- Resource Type
- Journal article