Journal article
Anaplasma marginale Type IV Secretion System Proteins VirB2, VirB7, VirB11, and VirD4 Are Immunogenic Components of a Protective Bacterial Membrane Vaccine
Infection and immunity, Vol.78(3), pp.1314-1325
03/2010
Handle:
https://hdl.handle.net/2376/117813
PMCID: PMC2825951
PMID: 20065028
Abstract
Anaplasma
and related
Ehrlichia
spp. are important tick-borne, Gram-negative bacterial pathogens of livestock and humans that cause acute infection and disease and can persist. Immunization of cattle with an
Anaplasma marginale
fraction enriched in outer membranes (OM) can provide complete protection against disease and persistent infection. Serological responses of OM vaccinees to the OM proteome previously identified over 20 antigenic proteins, including three type IV secretion system (T4SS) proteins, VirB9-1, VirB9-2, and VirB10. Subsequent studies showed that these three proteins also stimulated CD4
+
T-cell responses in OM vaccinees. The T4SS, composed of a complex of proteins spanning the inner and outer membranes of certain bacteria, is an important virulence factor but is relatively unexplored as a vaccine target. The goal of this study was to determine if additional T4SS proteins are immunogenic for animals immunized with the protective OM fraction of
A. marginale.
T4SS proteins expressed by
in vitro
transcription and translation were screened for stimulating proliferation of T cells from OM vaccinees, and immunogenic proteins were expressed as recombinant proteins in
Escherichia coli
and their immunogenicity was verified. VirB2, a putative VirB7, VirB11, and VirD4 were immunogenic for OM vaccinees expressing several common major histocompatibility complex (MHC) class II haplotypes. VirB2 is encoded by multiple genes that share a conserved central region, and epitope mapping revealed T-cell epitopes in this region. The discovery of novel immunogenic T4SS proteins recognized by outbred individuals with common MHC haplotypes further justifies evaluating the T4SS as a potential vaccine candidate for pathogenic bacteria.
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Details
- Title
- Anaplasma marginale Type IV Secretion System Proteins VirB2, VirB7, VirB11, and VirD4 Are Immunogenic Components of a Protective Bacterial Membrane Vaccine
- Creators
- Eric L Sutten - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164Junzo Norimine - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164Paul A Beare - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164Robert A Heinzen - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164Job E Lopez - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164Kaitlyn Morse - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164Kelly A Brayton - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164Joseph J Gillespie - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164Wendy C Brown - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164
- Publication Details
- Infection and immunity, Vol.78(3), pp.1314-1325
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- American Society for Microbiology (ASM)
- Identifiers
- 99900547820601842
- Language
- English
- Resource Type
- Journal article