Androgen-regulated transcripts in the neonatal mouse testis as determined through microarray analysis

Qing Zhou; James E Shima; Rong Nie; Patrick J Friel; Michael D Griswold

doi:10.1095/biolreprod.104.035915

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Androgen-regulated transcripts in the neonatal mouse testis as determined through microarray analysis

Journal article

Open access

Peer reviewed

Androgen-regulated transcripts in the neonatal mouse testis as determined through microarray analysis

Qing Zhou, James E Shima, Rong Nie, Patrick J Friel and Michael D Griswold

Biology of reproduction, Vol.72(4), pp.1010-1019

04/2005

DOI: https://doi.org/10.1095/biolreprod.104.035915

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https://hdl.handle.net/2376/108757

PMID: 15601916

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Abstract

Animals, Newborn

Cell Division - genetics

Testosterone Propionate - pharmacology

Gene Expression - drug effects

Oligonucleotide Array Sequence Analysis

Testis - cytology

RNA, Messenger - genetics

Cells, Cultured

Androgen Antagonists - pharmacology

Male

Flutamide - pharmacology

Sertoli Cells - drug effects

Testis - drug effects

Testis - physiology

Cell Differentiation - genetics

Sertoli Cells - physiology

Animals

Receptors, Androgen - genetics

Androgens - pharmacology

Sertoli Cells - cytology

Gene Expression - physiology

Biomarkers

Dihydrotestosterone - pharmacology

Mice

Androgens are required for normal spermatogenesis in mammalian testes. These hormones directly regulate testicular somatic cells that, in turn, support germ cell differentiation. However, the identity of genes under androgen regulation in the testis are not well known. In the present study, neonatal male mice (8 days postpartum) treated by testosterone propionate (TP) were used to study androgen action in the testis as evidenced by alterations in gene expression. Mice were treated with 0.5 mg of TP or dihydrotestosterone (DHT) or vehicle (oil), and testes were harvested 4, 8, and 16 h after treatment. Global gene expression was monitored by microarray analysis. Real-time reverse transcription-polymerase chain reaction was performed to confirm the microarray results. The methodology was verified by confirming the presence of previously characterized TP-regulated genes, including Pem in Sertoli cells and Cyp17a1 in Leydig cells. No significant differences in gene expression were found between TP- and DHT-treated samples. Microarray analysis identified 141, 119, and 109 up-regulated genes at 4, 8 and 16 h after TP treatment, respectively, and 83, 99, and 111 down-regulated genes at the same corresponding time points. The androgen regulation of the selected gene was verified further using testes from flutamide-treated adult mice and isolated Sertoli cells in culture. The data generated in the present study may serve as a foundation for hypothesis-driven research and provide insights regarding gene networks and pathways under androgen control in the testis.

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Title: Androgen-regulated transcripts in the neonatal mouse testis as determined through microarray analysis
Creators: Qing Zhou - Center for Reproductive Biology, School of Molecular Biosciences, Washington State University, Pullman, Washington 99164, USA
James E Shima
Rong Nie
Patrick J Friel
Michael D Griswold
Publication Details: Biology of reproduction, Vol.72(4), pp.1010-1019
Academic Unit: Molecular Biosciences, School of
Publisher: United States
Grant note: U54 HD42454 / NICHD NIH HHS HD10808 / NICHD NIH HHS
Identifiers: 99900547036701842
Language: English
Resource Type: Journal article