Journal article
Angiotensin IV AT4-receptor system in the rat kidney
American journal of physiology. Renal physiology, Vol.274(2), pp.F290-F299
02/01/1998
Handle:
https://hdl.handle.net/2376/100269
PMID: 9486224
Abstract
Angiotensin IV, {[des-Asp1,Arg2]ANG II or ANG-(3—8)}, has been shown to preferentially bind to a novel angiotensin binding site (AT4receptor). The cellular location and function of this receptor in the rat kidney is unknown. Autoradiography localized AT4 receptors to the cell body and apical membrane of convoluted and straight proximal tubules in the cortex and outer stripe of the outer medulla. ANG IV (0.1 pM-1 μM) elicited a concentration-dependent decrease in transcellular Na+ transport (as measured by proximal tubule O2 consumption rates) in fresh suspensions of control or nystatin-stimulated (bypasses rate-limiting step of apical Na+entry) rat proximal tubules. The inhibitory effect of 1 pM ANG IV was unaltered by either 1 μM losartan (AT1-receptor antagonist) or 1 μM PD-123319 (AT2-receptor antagonist) and yet was abolished by 1 μM divalinal-ANG IV (AT4-receptor antagonist) or ouabain pretreatment. These results demonstrate that the kidney AT4-receptor system is localized to the proximal tubule and suggests that one potential biological role of this system is in the regulation of Na+ transport by inhibiting a ouabain-sensitive component of Na+-K+-adenosinetriphosphatase activity in the rat.
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Details
- Title
- Angiotensin IV AT4-receptor system in the rat kidney
- Creators
- Rajash K Handa - Department of Veterinary and Comparative Anatomy, Pharmacology and Physiology, College of Veterinary Medicine, Washington State University, Pullman, Washington 99164Luke T Krebs - Department of Veterinary and Comparative Anatomy, Pharmacology and Physiology, College of Veterinary Medicine, Washington State University, Pullman, Washington 99164Joseph W Harding - Department of Veterinary and Comparative Anatomy, Pharmacology and Physiology, College of Veterinary Medicine, Washington State University, Pullman, Washington 99164Shelly E Handa - Department of Veterinary and Comparative Anatomy, Pharmacology and Physiology, College of Veterinary Medicine, Washington State University, Pullman, Washington 99164
- Publication Details
- American journal of physiology. Renal physiology, Vol.274(2), pp.F290-F299
- Academic Unit
- Integrative Physiology and Neuroscience, Department of
- Identifiers
- 99900546533001842
- Language
- English
- Resource Type
- Journal article