Journal article
Antibacterial efficacy of core-shell nanostructures encapsulating gentamicin against an in vivo intracellular Salmonella model
International journal of nanomedicine, Vol.4, pp.289-297
2009
Handle:
https://hdl.handle.net/2376/114573
PMID: 20054433
Abstract
Pluronic based core-shell nanostructures encapsulating gentamicin were designed in this study. Block copolymers of (PAA
−+
Na-
b
-(PEO-
b
-PPO-
b
-PEO)-b-PAA
−+
Na) were blended with PAA
−
Na
+
and complexed with the polycationic antibiotic gentamicin to form nanostructures. Synthesized nanostructures had a hydrodynamic diameter of 210 nm, zeta potentials of −0.7 (±0.2), and incorporated ∼20% by weight of gentamicin. Nanostructures upon co-incubation with J774A.1 macrophage cells showed no adverse toxicity
in vitro
. Nanostructures administered
in vivo
either at multiple dosage of 5 μg g
−1
or single dosage of 15 μg g
−1
in AJ-646 mice infected with
Salmonella
resulted in significant reduction of viable bacteria in the liver and spleen. Histopathological evaluation for concentration-dependent toxicity at a dosage of 15 μg g
−1
revealed mineralized deposits in 50% kidney tissues of free gentamicin-treated mice which in contrast was absent in nanostructure-treated mice. Thus, encapsulation of gentamicin in nanostructures may reduce toxicity and improve
in vivo
bacterial clearance.
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Details
- Title
- Antibacterial efficacy of core-shell nanostructures encapsulating gentamicin against an in vivo intracellular Salmonella model
- Creators
- Ashish Ranjan - Department of Large Animal Clinical SciencesNikorn Pothayee - Institute for Critical Technology and Applied ScienceMohammed N Seleem - Institute for Critical Technology and Applied ScienceRonald D Tyler - Department of Biomedical Sciences and Pathobiology, Virginia Polytechnic Institute and State University, Blacksburg, VABonnie Brenseke - Department of Biomedical Sciences and Pathobiology, Virginia Polytechnic Institute and State University, Blacksburg, VANammalwar Sriranganathan - Institute for Critical Technology and Applied ScienceJudy S Riffle - Institute for Critical Technology and Applied ScienceRamanathan Kasimanickam - Department of Large Animal Clinical Sciences
- Publication Details
- International journal of nanomedicine, Vol.4, pp.289-297
- Publisher
- Dove Medical Press
- Identifiers
- 99900548299101842
- Language
- English
- Resource Type
- Journal article