Journal article
Antibody against an Anaplasma marginale MSP5 epitope common to tick and erythrocyte stages identifies persistently infected cattle
Journal of clinical microbiology, Vol.34(9), pp.2225-2230
09/1996
Handle:
https://hdl.handle.net/2376/109389
PMCID: PMC229221
PMID: 8862589
Abstract
A protein epitope of major surface protein 5 (MSP5), defined by monoclonal antibody (MAb) ANAF16C1, is conserved among Anaplasma species (E. S. Visser, T. C. McGuire, G. H. Palmer, W. C. Davis, V. Shkap, E. Pipano, and D. P. Knowles, Jr., Infect. Immun. 60:5139-5144, 1992) and is expressed in the salivary glands of infected ticks. A competitive inhibition ELISA (cELISA) for the detection of bovine anti-MSP5 antibodies was developed by using purified recombinant MSP5 fusion protein and MAb ANAF16C1. The specificity of the recombinant-MSP5 cELISA within North America was established by using 261 serum samples from cattle in the regions of Hawaii and Northern Ontario where anaplasmosis is not endemic and from cattle proven by splenectomy or subinoculation of whole blood into susceptible splenectomized recipients to be uninfected. The maximum percent inhibition by these sera was 18%. Sera known to be positive were obtained from 35 cattle either experimentally inoculated with infected erythrocytes or exposed to infected Dermacentor andersoni ticks. Thirty-four of the 35 serum samples inhibited MAb ANAF16C1 binding by > or = 25%. During acute infection, the MSP5 cELISA detected antibodies prior to or concomitantly with the appearance of rickettsiae in erythrocytes. Antibodies were detectable in sera from persistently infected cattle inoculated as long as 6 years previously.
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Details
- Title
- Antibody against an Anaplasma marginale MSP5 epitope common to tick and erythrocyte stages identifies persistently infected cattle
- Creators
- D Knowles - Animal Disease Research Unit, USDA Agricultural Research Service, Pullman, Washington, USA. dknowles@vctmed.wsu.eduS Torioni de Echaide - Animal Disease Research Unit, USDA Agricultural Research Service, Pullman, Washington, USA. dknowles@vctmed.wsu.eduG Palmer - Animal Disease Research Unit, USDA Agricultural Research Service, Pullman, Washington, USA. dknowles@vctmed.wsu.eduT McGuire - Animal Disease Research Unit, USDA Agricultural Research Service, Pullman, Washington, USA. dknowles@vctmed.wsu.eduD Stiller - Animal Disease Research Unit, USDA Agricultural Research Service, Pullman, Washington, USA. dknowles@vctmed.wsu.eduT McElwain - Animal Disease Research Unit, USDA Agricultural Research Service, Pullman, Washington, USA. dknowles@vctmed.wsu.edu
- Publication Details
- Journal of clinical microbiology, Vol.34(9), pp.2225-2230
- Academic Unit
- Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
- Identifiers
- 99900546919701842
- Language
- English
- Resource Type
- Journal article