Journal article
Antigen-specific proliferation and activation of peripheral blood mononuclear cells from Mycobacterium bovis-infected reindeer
Veterinary immunology and immunopathology, Vol.111(3), pp.263-277
2006
Handle:
https://hdl.handle.net/2376/109207
PMID: 16584788
Abstract
To evaluate antigen-specific proliferative and activation-associated responses from
Mycobacterium bovis-infected reindeer, blood mononuclear cells from
M. bovis- (
n
=
10) and non-infected reindeer (
n
=
4) were stimulated with a recombinant early secretory antigenic target-6 and culture filtrate protein-10 fusion protein (rESAT6:CFP10),
M. bovis purified protein derivative, pokeweed mitogen, or medium alone and evaluated by flow cytometry using dye tracker analysis and cell surface marker staining. γδ TCR
+ and CD8
+ cells, but not CD4
+ cells, from
M. bovis-infected reindeer proliferated in response to specific antigen stimulation. Expression (i.e., mean fluorescence intensity) of CD44 was increased and CD62L decreased on proliferative as compared to non-proliferative fractions in antigen- and mitogen-stimulated cultures. In response rESAT6:CFP10 stimulation, MHC II fluorescence intensity was increased on CD4
+, γδ TCR
+, CD172a
+, and IgM
+ cells from infected reindeer as compared to that of non-stimulated cells from the same reindeer. Recombinant ESAT6:CFP10 stimulation also induced expansion of a CD172a
+, MHC II
+ population within mononuclear cell cultures from
M. bovis-infected reindeer. Despite a moderate challenge dose and extended duration of incubation, experimental infection of reindeer was generally limited to lymph nodes draining the inoculation site, suggestive of host resistance to progressive disease. Present in vitro findings, therefore, may be predictive of host responses by reindeer that limit progression to disseminated disease.
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Details
- Title
- Antigen-specific proliferation and activation of peripheral blood mononuclear cells from Mycobacterium bovis-infected reindeer
- Creators
- W. Ray Waters - Bacterial Diseases of Livestock Research Unit, National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, 2300 Dayton Avenue, P.O. Box 70, Ames, IA 50010, United StatesMitchell V Palmer - Bacterial Diseases of Livestock Research Unit, National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, 2300 Dayton Avenue, P.O. Box 70, Ames, IA 50010, United StatesTyler C Thacker - Bacterial Diseases of Livestock Research Unit, National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, 2300 Dayton Avenue, P.O. Box 70, Ames, IA 50010, United StatesF Chris Minion - Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, IA 50011, United StatesWilliam C Davis - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, United States
- Publication Details
- Veterinary immunology and immunopathology, Vol.111(3), pp.263-277
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900547247701842
- Language
- English
- Resource Type
- Journal article