Antigenic structure of soluble herpes simplex virus (HSV) glycoprotein D correlates with inhibition of HSV infection

A V Nicola; C Peng; H Lou; G H Cohen; R J Eisenberg

doi:10.1128/JVI.71.4.2940-2946.1997

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Antigenic structure of soluble herpes simplex virus (HSV) glycoprotein D correlates with inhibition of HSV infection

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Antigenic structure of soluble herpes simplex virus (HSV) glycoprotein D correlates with inhibition of HSV infection

A V Nicola, C Peng, H Lou, G H Cohen and R J Eisenberg

Journal of virology, Vol.71(4), pp.2940-2946

04/1997

DOI: https://doi.org/10.1128/JVI.71.4.2940-2946.1997

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https://hdl.handle.net/2376/111510

PMCID: PMC191422

PMID: 9060653

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Abstract

Recombinant Fusion Proteins - immunology

Cell Line

Herpesvirus 2, Human - isolation & purification

Spodoptera - cytology

Viral Envelope Proteins - genetics

Species Specificity

Humans

Solubility

Cercopithecus aethiops

Viral Plaque Assay

Antigens, Viral - chemistry

Antigens, Viral - genetics

Recombinant Fusion Proteins - chemistry

Antigens, Viral - immunology

Animals

Herpesvirus 2, Human - immunology

Viral Envelope Proteins - chemistry

Recombinant Fusion Proteins - genetics

Herpesvirus 1, Human - immunology

Viral Envelope Proteins - immunology

Vero Cells

Herpesvirus 1, Human - isolation & purification

Soluble forms of herpes simplex virus (HSV) glycoprotein D (gD) block viral penetration. Likewise, most HSV strains are sensitive to gD-mediated interference by cells expressing gD. The mechanism of both forms of gD-mediated inhibition is thought to be at the receptor level. We analyzed the ability of different forms of soluble, truncated gD (gDt) to inhibit infection by different strains of HSV-1 and HSV-2. Strains that were resistant to gD-mediated interference were also resistant to inhibition by gDt, thereby suggesting a link between these two phenomena. Virion gD was the major viral determinant for resistance to inhibition by gDt. An insertion-deletion mutant, gD-1(delta 290-299t), had an enhanced inhibitory activity against most strains tested. The structure and function of gDt proteins derived from the inhibition-resistant viruses rid1 and ANG were analyzed. gD-1(ridlt) and gD-1(ANGt) had a potent inhibitory effect on plaque formation by wild-type strains of HSV but, surprisingly, little or no effect on their parental strains. As measured by quantitative enzyme-linked immunosorbent assay with a diverse panel of monoclonal antibodies, the antigenic structures of gD-1(rid1t) and gD-1(ANGt) were divergent from that of the wild type yet were similar to each other and to that of gD-1 (delta 290-299t). Thus, three different forms of gD have common antigenic changes that correlate with enhanced inhibitory activity against HSV. We conclude that inhibition of HSV infectivity by soluble gD is influenced by the antigenic conformation of the blocking gDt as well as the form of gD in the target virus.

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Title: Antigenic structure of soluble herpes simplex virus (HSV) glycoprotein D correlates with inhibition of HSV infection
Creators: A V Nicola - Department of Microbiology, University of Pennsylvania, Philadelphia 19104, USA. anicola@biochem.dental.upenn.edu
C Peng
H Lou
G H Cohen
R J Eisenberg
Publication Details: Journal of virology, Vol.71(4), pp.2940-2946
Academic Unit: Veterinary Microbiology and Pathology, Department of
Publisher: United States
Grant note: NS-30606 / NINDS NIH HHS AI-18289 / NIAID NIH HHS DE-08239 / NIDCR NIH HHS
Identifiers: 99900547427001842
Language: English
Resource Type: Journal article