Journal article
Antisense and sense expression of cDNA coding for CYP73A15, a class II cinnamate 4-hydroxylase, leads to a delayed and reduced production of lignin in tobacco
Phytochemistry (Oxford), Vol.57(7), pp.1159-1166
2001
Handle:
https://hdl.handle.net/2376/106551
PMID: 11430988
Abstract
A number of plant species contain the class II of genes encoding the cytochrome P450, CYP73, the cognate protein of which cinnamic acid 4-hydroxylase, is the second enzyme of the phenylpropanoid pathway. In order to begin to determine possible functionality, tobacco has been transformed with a truncated French bean class II cinnamate hydroxylase (CYP73A15) in the sense and antisense orientations. Signals for C4H protein could be detected in vascular tissue from wild-type plants using heterologous probes. The transformed plants showed a normal phenotype, even though detectable C4H protein was much reduced in tissue prints. Young propagated transformants displayed a range of reduced C4H activities, as well as either reduced or no phloroglucinol-stainable lignin. However, all mature tobacco plants showed the accumulation of lignin, even though its deposition was apparently delayed. This was not due to induction of tyrosine ammonia-lyase activity, which was not detected, but instead it is presumed due to sufficient C4H residual activity. Analysis of the lignin content of the plants showed reductions of up to 30% with a slightly reduced syringyl to guaiacyl ratio as compared to wild type. This reduction level was favourable in comparison with some other targets in the lignification pathway that have been manipulated including that of class I cinnamate 4-hydroxylase. It is proposed that the class II cinnamate 4-hydroxylase might also function in lignification in a number of species including French bean and tobacco, based on these data.
Tobacco plants antisense or partial sense manipulated for a class II C4H show little or no lignin in young stems and reduced lignin content in mature internodes.
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Details
- Title
- Antisense and sense expression of cDNA coding for CYP73A15, a class II cinnamate 4-hydroxylase, leads to a delayed and reduced production of lignin in tobacco
- Creators
- Kristopher Blee - Division of Biochemistry, School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey, TW20 0EX, UKJoon W Choi - Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, USAAnn P O’Connell - Division of Biochemistry, School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey, TW20 0EX, UKSteve C Jupe - Division of Biochemistry, School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey, TW20 0EX, UKWolfgang Schuch - Zeneca Wheat Improvement Centre, Norwich, NR4 7UH, UKNorman G Lewis - Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, USAG.Paul Bolwell - Division of Biochemistry, School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey, TW20 0EX, UK
- Publication Details
- Phytochemistry (Oxford), Vol.57(7), pp.1159-1166
- Academic Unit
- Biological Chemistry, Institute of
- Publisher
- Elsevier Ltd
- Identifiers
- 99900546838201842
- Language
- English
- Resource Type
- Journal article