Journal article
Apoferritin-templated yttrium phosphate nanoparticle conjugates for radioimmunotherapy of cancers
Journal of nanoscience and nanotechnology, Vol.8(5), pp.2316-2322
05/2008
Handle:
https://hdl.handle.net/2376/117351
PMID: 18572643
Abstract
We report a templated-synthetic approach based on protein-cage of apoferritin to prepare radionuclide nanoparticle (NP) conjugates. Non-radioactive yttrium (89Y) was used as a model target and surrogate for radioyttrium (90Y) to prepare the nanoparticle conjugate. The center cavity and multiple channel structure of apoferritin offer a fast and facile method to precipitate yttrium phosphate by diffusing yttrium and phosphate ions into the cavity of apoferritin, resulting a core-shell nanoparticle. The yttrium phosphate/apoferritin nanoparticle was functionalized with biotin for further application. The synthesized nanoparticle was characterized by transmission electron microscopy (TEM) and X-ray photoelectron spectroscopy (XPS). We found that the resulting nanoparticles were uniform in size, with a diameter of around 8 nm. We tested the pre-targeting capability of the biotin-modified yttrium phosphate/apoferritin nanoparticle conjugate with streptavidin-modified magnetic beads and with aid of streptavidin-modified fluorescein isothiocyanate (FITC) tracer. This work shows that an yttrium phosphate NP conjugate provides a fast, simple and efficient method to prepare radioactive yttrium conjugate for potential applications in radioimmunotherapy of cancer.
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Details
- Title
- Apoferritin-templated yttrium phosphate nanoparticle conjugates for radioimmunotherapy of cancers
- Creators
- Hong Wu - Pacific Northwest National Laboratory, Richland, WA 99352, USAJun WangZhemin WangDarrell R FisherYuehe Lin
- Publication Details
- Journal of nanoscience and nanotechnology, Vol.8(5), pp.2316-2322
- Academic Unit
- Mechanical and Materials Engineering, School of
- Publisher
- United States
- Grant note
- U01 NS058161-01 / NINDS NIH HHS
- Identifiers
- 99900547856801842
- Language
- English
- Resource Type
- Journal article