Journal article
Application of Cydia pomonella expressed sequence tags: Identification and expression of three general odorant binding proteins in codling moth
Insect science, Vol.20(5), pp.559-574
10/2013
Handle:
https://hdl.handle.net/2376/113963
PMCID: PMC4255946
PMID: 23956229
Abstract
The codling moth,
Cydia pomonella
, is one of the most important pests of pome fruits in the world, yet the molecular genetics and the physiology of this insect remain poorly understood. A combined assembly of 8 341 expressed sequence tags was generated from Roche 454 GS-FLX sequencing of eight tissue-specific cDNA libraries. Putative chemosensory proteins (12) and odorant binding proteins (OBPs) (18) were annotated, which included three putative general OBP (GOBP), one more than typically reported for other Lepidoptera. To further characterize CpomGOBPs, we cloned cDNA copies of their transcripts and determined their expression patterns in various tissues. Cloning and sequencing of the 698 nt transcript for CpomGOBP1 resulted in the prediction of a 163 amino acid coding region, and subsequent RT-PCR indicated that the transcripts were mainly expressed in antennae and mouthparts. The 1 289 nt (160 amino acid) CpomGOBP2 and the novel 702 nt (169 amino acid) CpomGOBP3 transcripts are mainly expressed in antennae, mouthparts, and female abdomen tips. These results indicate that next generation sequencing is useful for the identification of novel transcripts of interest, and that codling moth expresses a transcript encoding for a new member of the GOBP subfamily.
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Details
- Title
- Application of Cydia pomonella expressed sequence tags: Identification and expression of three general odorant binding proteins in codling moth
- Creators
- Stephen F Garczynski - USDA-ARS, Yakima Agricultural Research Laboratory, Wapato, WA 98951Brad S Coates - USDA-ARS, Corn Insect and Crop Genetics Research Unit, Genetics Laboratory, Iowa State University, Ames, IA 50011Thomas R Unruh - USDA-ARS, Yakima Agricultural Research Laboratory, Wapato, WA 98951Scott Schaeffer - Department of Horticulture and Landscape Architecture, Washington State University, Pullman, WA 99164, USADerick Jiwan - Department of Horticulture and Landscape Architecture, Washington State University, Pullman, WA 99164, USATyson Koepke - Department of Horticulture and Landscape Architecture, Washington State University, Pullman, WA 99164, USAAmit Dhingra - Department of Horticulture and Landscape Architecture, Washington State University, Pullman, WA 99164, USA
- Publication Details
- Insect science, Vol.20(5), pp.559-574
- Academic Unit
- Crop and Soil Sciences, Department of; Horticulture, Department of
- Identifiers
- 99900548258601842
- Language
- English
- Resource Type
- Journal article