Journal article
Application of TaqMan Low-Density Arrays for Simultaneous Detection of Multiple Respiratory Pathogens
Journal of clinical microbiology, Vol.49(6), pp.2175-2182
06/2011
Handle:
https://hdl.handle.net/2376/108712
PMCID: PMC3122721
PMID: 21471348
Abstract
The large and growing number of viral and bacterial pathogens responsible for respiratory infections poses a challenge for laboratories seeking to provide rapid and comprehensive pathogen identification. We evaluated a novel application of the TaqMan low-density array (TLDA) cards for real-time PCR detection of 21 respiratory-pathogen targets. The performance of the TLDA was compared to that of individual real-time PCR (IRTP) assays with the same primers and probes using (i) nucleic acids extracted from the 21 pathogen strains and 66 closely related viruses and bacteria and (ii) 292 clinical respiratory specimens. With spiked samples, TLDA cards were about 10-fold less sensitive than IRTP assays. By using 292 clinical specimens to generate 2,238 paired individual assays, the TLDA card exhibited 89% sensitivity (95% confidence interval [CI], 86 to 92%; range per target, 47 to 100%) and 98% specificity (95% CI, 97 to 99%; range per target, 85 to 100%) overall compared to IRTP assays as the gold standard with a threshold cycle (
C
T
) cutoff of 43. The TLDA card approach offers promise for rapid and simultaneous identification of multiple respiratory pathogens for outbreak investigations and disease surveillance.
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Details
- Title
- Application of TaqMan Low-Density Arrays for Simultaneous Detection of Multiple Respiratory Pathogens
- Creators
- Maja Kodani - Division of Bacterial DiseasesGenyan Yang - Division of Bacterial DiseasesLaura M Conklin - Division of Bacterial DiseasesTatiana C Travis - Division of Bacterial DiseasesCynthia G Whitney - Division of Bacterial DiseasesLarry J Anderson - Division of Viral DiseasesStephanie J Schrag - Division of Bacterial DiseasesThomas H Taylor - Division of Bacterial DiseasesBernard W Beall - Division of Bacterial DiseasesRobert F Breiman - Office of The DirectorDaniel R Feikin - Division of Emerging Infections and Surveillance Services, National Center for Preparedness, Detection and Control of Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333M. Kariuki Njenga - Division of Global Disease Detection and Emergency Response, Center for Global HealthLeonard W Mayer - Division of Bacterial DiseasesM. Steven Oberste - Division of Viral DiseasesMaria Lucia C Tondella - Division of Bacterial DiseasesJonas M Winchell - Division of Bacterial DiseasesStephen L Lindstrom - Influenza Division, National Center for Immunization and Respiratory DiseasesDean D Erdman - Division of Viral DiseasesBarry S Fields - Division of Bacterial Diseases
- Publication Details
- Journal of clinical microbiology, Vol.49(6), pp.2175-2182
- Academic Unit
- Paul G. Allen School for Global Animal Health
- Publisher
- American Society for Microbiology; 1752 N St., N.W., Washington, DC
- Identifiers
- 99900547359101842
- Language
- English
- Resource Type
- Journal article