Journal article
Application of chemisorption/desorption process of thiocholine for pesticide detection based on acetylcholinesterase biosensor
Sensors and actuators. B, Chemical, Vol.134(2), pp.908-912
2008
Handle:
https://hdl.handle.net/2376/107434
Abstract
One-step electrochemical deposition of gold nanoparticles (AuNPs) in chitosan hydrogel onto a planar gold electrode was used to create a favorable surface for the attachment of the enzyme acetylcholinesterase (AChE). AChE exhibited high affinity to its substrate of acetylthiocholine chloride (ATCl) from calculated Michealis–Menten constant (
K
m
app
) of 0.309
mM. Thiocholine, the product through AChE-catalyzed hydrolysis of ATCl, could be chemisorbed on the surface with an applied potential of −0.7
V and then desorbed in KOH solution, giving a measurable peak current. When enzymatic activity was inhibited by organophosphate pesticides, the amount of chemisorpted thiocholine on the interface was anticipated to decrease. As a result, a simple method for rapid determination of malathion was established based on the chemisorption/desorption process of thiocholine used as an indicator. Under the optimal conditions, the decrease in response was proportional to the concentration of malathion from 0.1 to 20
ng
mL
−1, with detection limit of 0.03
ng
mL
−1 (0.1
nM). The method based on one-step electrochemical deposition and chemisorption/desorption process of thiocholin has potential application in high-sensitive pesticide determinations.
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Details
- Title
- Application of chemisorption/desorption process of thiocholine for pesticide detection based on acetylcholinesterase biosensor
- Creators
- Dan Du - Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, ChinaJiawang Ding - Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, ChinaYuan Tao - Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, ChinaXi Chen - State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China
- Publication Details
- Sensors and actuators. B, Chemical, Vol.134(2), pp.908-912
- Academic Unit
- School of Mechanical and Materials Engineering
- Publisher
- Elsevier B.V
- Identifiers
- 99900547207301842
- Language
- English
- Resource Type
- Journal article