Journal article
Application of statistically-based experimental designs for the optimization of nisin production from whey
Biotechnology letters, Vol.25(11), pp.877-882
06/2003
Handle:
https://hdl.handle.net/2376/114413
PMID: 12889798
Abstract
Statistically-based experimental designs were applied for the optimization of nisin production by Lactococcus lactis in a whey-based medium. Yeast extract, KH2PO4, and MgSO4 were identified to have significant effects on nisin biosynthesis by a Plackett–Burman design. These three significant factors were subsequently optimized using central composite design, and the optimal conditions were determined to be 12.067 g l−1 for yeast extract, 0.569 g l−1 for KH2PO4, and 0.572 g l−1 for MgSO4. The validity of the optimal conditions was verified by a separate experiment.
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Details
- Title
- Application of statistically-based experimental designs for the optimization of nisin production from whey
- Creators
- Chuanbin Liu - Department of Biological Systems Engineering Washington State University Pullman WA 99164-6120 USAYan Liu - Department of Biological Systems Engineering Washington State University Pullman WA 99164-6120 USAWei Liao - Department of Biological Systems Engineering Washington State University Pullman WA 99164-6120 USAZhiyou Wen - Department of Biological Systems Engineering Washington State University Pullman WA 99164-6120 USAShulin Chen - Department of Biological Systems Engineering Washington State University Pullman WA 99164-6120 USA
- Publication Details
- Biotechnology letters, Vol.25(11), pp.877-882
- Academic Unit
- Biological Systems Engineering, Department of; Plant Pathology, Department of
- Publisher
- Kluwer Academic Publishers; Dordrecht
- Identifiers
- 99900547661601842
- Language
- English
- Resource Type
- Journal article