Journal article
Application of the Enfer chemiluminescent multiplex ELISA system for the detection of Mycobacterium bovis infection in goats
Veterinary microbiology, Vol.154(3), pp.292-297
2012
Handle:
https://hdl.handle.net/2376/109937
PMID: 21862242
Abstract
A study was conducted to optimise a multiplex serological immunoassay for use in identification of goats infected with
Mycobacterium bovis. To assess assay specificity, 31 goats with a history of being free from
M. bovis infection were used. To determine assay sensitivity, 180 Single Intradermal Comparative Tuberculin test (SICTT) positive goats were recruited. Additionally, 286 SICTT negative goats classed as potentially exposed animals present in the same positive herds were also included in the study. The results of the assay demonstrated a specificity of 100%. The multiplex assay detected 57/60 SICTT (95.0%) positive animals in one
M. bovis infected herd and 120/120 (100%) in a second herd. In a separate experiment, 28
M. caprae culture confirmed infected goats from Spain were assayed, of which 24 (85.7%) were found positive in the test. The results show that inclusion of an antibody based assay can improve the ability to identify
M. bovis and
M. caprae infected goats. With further development and validation the multiplex assay may prove to be a useful tool for control of
M. bovis and
M. caprae infection in goats.
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Details
- Title
- Application of the Enfer chemiluminescent multiplex ELISA system for the detection of Mycobacterium bovis infection in goats
- Creators
- Eduard Shuralev - Enfer Scientific, Unit T, M7 Business Park, Newhall, Naas, Co. Kildare, IrelandPadraig Quinn - Enfer Scientific, Unit T, M7 Business Park, Newhall, Naas, Co. Kildare, IrelandMairead Doyle - School of Agriculture, Food Science and Veterinary Medicine, University College Dublin, Dublin, IrelandAnthony Duignan - Department of Agriculture, Fisheries and Food, Kildare Street, Dublin, IrelandHang Fai Kwok - Fusion Antibodies Ltd., Springbank Industrial Estate, Belfast, Northern Ireland BT17 0QL, UKJavier Bezos - Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense de Madrid, Avda. Puerta de Hierro s/n, 28040 Madrid, SpainShane A Olwill - Fusion Antibodies Ltd., Springbank Industrial Estate, Belfast, Northern Ireland BT17 0QL, UKEamonn Gormley - School of Agriculture, Food Science and Veterinary Medicine, University College Dublin, Dublin, IrelandAlicia Aranaz - Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense de Madrid, Avda. Puerta de Hierro s/n, 28040 Madrid, SpainMargaret Good - Department of Agriculture, Fisheries and Food, Kildare Street, Dublin, IrelandWilliam C Davis - Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, USAJohn Clarke - Enfer Scientific, Unit T, M7 Business Park, Newhall, Naas, Co. Kildare, IrelandClare Whelan - Enfer Scientific, Unit T, M7 Business Park, Newhall, Naas, Co. Kildare, Ireland
- Publication Details
- Veterinary microbiology, Vol.154(3), pp.292-297
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900546909501842
- Language
- English
- Resource Type
- Journal article