Arabidopsis AXR6 encodes CUL1 implicating SCF E3 ligases in auxin regulation of embryogenesis

Hanjo Hellmann; Lawrence Hobbie; Anngela Chapman; Sunethra Dharmasiri; Nihal Dharmasiri; Carlos del Pozo; Didier Reinhardt; Mark Estelle

doi:10.1093/emboj/cdg335

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Arabidopsis AXR6 encodes CUL1 implicating SCF E3 ligases in auxin regulation of embryogenesis

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Arabidopsis AXR6 encodes CUL1 implicating SCF E3 ligases in auxin regulation of embryogenesis

Hanjo Hellmann, Lawrence Hobbie, Anngela Chapman, Sunethra Dharmasiri, Nihal Dharmasiri, Carlos del Pozo, Didier Reinhardt and Mark Estelle

The EMBO journal, Vol.22(13), pp.3314-3325

07/01/2003

DOI: https://doi.org/10.1093/emboj/cdg335

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https://hdl.handle.net/2376/101216

PMCID: PMC165659

PMID: 12839993

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Abstract

IAA

auxin

cullin

Arabidopsis

SCF

The AXR6 gene is required for auxin signaling in the Arabidopsis embryo and during postembryonic development. One of the effects of auxin is to stimulate degradation of the Aux/IAA auxin response proteins through the action of the ubiquitin protein ligase SCF TIR1 . Here we show that AXR6 encodes the SCF subunit CUL1. The axr6 mutations affect the ability of mutant CUL1 to assemble into stable SCF complexes resulting in reduced degradation of the SCF TIR1 substrate AXR2/IAA7. In addition, we show that CUL1 is required for lateral organ initiation in the shoot apical meristem and the inflorescence meristem. These results indicate that the embryonic axr6 phenotype is related to a defect in SCF function and accumulation of Aux/IAA proteins such as BDL/IAA12. In addition, we show that CUL1 has a role in auxin response throughout the life cycle of the plant.

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Title: Arabidopsis AXR6 encodes CUL1 implicating SCF E3 ligases in auxin regulation of embryogenesis
Creators: Hanjo Hellmann - Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, TX 78712
Lawrence Hobbie - Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, TX 78712
Anngela Chapman - Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, TX 78712
Sunethra Dharmasiri - Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, TX 78712
Nihal Dharmasiri - Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, TX 78712
Carlos del Pozo - Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, TX 78712
Didier Reinhardt - Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, TX 78712
Mark Estelle - Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, TX 78712
Publication Details: The EMBO journal, Vol.22(13), pp.3314-3325
Academic Unit: Biological Sciences, School of
Publisher: Oxford University Press; Oxford, UK
Identifiers: 99900546660001842
Language: English
Resource Type: Journal article