Journal article
Arabidopsis DDB1a and DDB1b are critical for embryo development
Planta, Vol.232(3), pp.555-566
08/2010
Handle:
https://hdl.handle.net/2376/101411
PMID: 20499085
Abstract
DNA Damaged binding protein 1 (DDB1) is a highly conserved protein of around 125 kDa. It serves as a substrate adaptor subunit to a CUL4-based E3 ubiquitin ligase within the ubiquitin proteasome pathway. However, based on a set of three beta-propellers, the protein is able to mediate various protein-protein interactions, suggesting that it participates in many developmental and physiological processes in the plant. Arabidopsis encodes for two closely related DDB1 proteins, named DDB1a and DDB1b. While loss-of DDB1a does not severely affect development, loss-of DDB1b has been reported to result in an embryo lethal phenotype. Here we describe two novel ddb1b T-DNA insertion mutants that are not embryo lethal, which we utilized as genetic tools to dissect DDB1b from DDB1a function. Information generated by these studies showed that the C-terminal part of the DDB1 proteins is critical for specific protein-protein interactions. In addition, we demonstrated that DDB1a, like DDB1b, is critical for embryo development, and that both proteins have distinct functions in whole plant development.
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Details
- Title
- Arabidopsis DDB1a and DDB1b are critical for embryo development
- Creators
- Anne Bernhardt - Freie Universität, Berlin, GermanySutton MooneyHanjo Hellmann
- Publication Details
- Planta, Vol.232(3), pp.555-566
- Academic Unit
- Biological Sciences, School of
- Publisher
- Germany
- Identifiers
- 99900546681601842
- Language
- English
- Resource Type
- Journal article