Journal article
Arabidopsis LATERAL ORGAN BOUNDARIES negatively regulates brassinosteroid accumulation to limit growth in organ boundaries
Proceedings of the National Academy of Sciences - PNAS, Vol.109(51), pp.21146-21151
12/18/2012
Handle:
https://hdl.handle.net/2376/105308
PMCID: PMC3529045
PMID: 23213252
Abstract
Leaves and flowers begin life as outgrowths from the edges of shoot apical meristems. Stem cell divisions in the meristem center replenish cells that are incorporated into organ primordia at the meristem periphery and leave the meristem. Organ boundaries, regions of limited growth that separate forming organs from the meristem, serve to isolate these two domains and are critical for coordination of organogenesis and meristem maintenance. Boundary formation and maintenance are poorly understood processes, despite the identification of a number of boundary-specific transcription factors. Here we provide genetic and biochemical evidence that the
Arabidopsis thaliana
transcription factor LATERAL ORGAN BOUNDARIES (LOB) negatively regulates accumulation of the plant steroid hormone brassinosteroid (BR) in organ boundaries. We found that ectopic expression of
LOB
results in reduced BR responses. We identified
BAS1
, which encodes a BR-inactivating enzyme, as a direct target of LOB transcriptional activation. Loss-of-function
lob
mutants exhibit organ fusions, and this phenotype is suppressed by expression of
BAS1
under the
LOB
promoter, indicating that BR hyperaccumulation contributes to the
lob
mutant phenotype. In addition,
LOB
expression is BR regulated; therefore, LOB and BR form a feedback loop to modulate local BR accumulation in organ boundaries to limit growth in the boundary domain.
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Details
- Title
- Arabidopsis LATERAL ORGAN BOUNDARIES negatively regulates brassinosteroid accumulation to limit growth in organ boundaries
- Creators
- Elizabeth M Bell - Department of Botany and Plant Sciences, Center for Plant Cell BiologyWan-ching Lin - Department of Botany and Plant Sciences, Center for Plant Cell BiologyAman Y Husbands - Department of Botany and Plant Sciences, Center for Plant Cell BiologyLifeng Yu - Department of Botany and Plant Sciences, Center for Plant Cell BiologyVenkateswari Jaganatha - Department of Botany and Plant Sciences, Center for Plant Cell BiologyBarbara Jablonska - Department of Botany and Plant Sciences, Center for Plant Cell BiologyAmanda Mangeon - Department of Botany and Plant Sciences, Center for Plant Cell BiologyMichael M Neff - Department of Crop and Soil SciencesThomas Girke - Department of Botany and Plant Sciences, Center for Plant Cell BiologyPatricia S Springer - Department of Botany and Plant Sciences, Center for Plant Cell Biology
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, Vol.109(51), pp.21146-21151
- Academic Unit
- Crop and Soil Sciences, Department of
- Publisher
- National Academy of Sciences
- Identifiers
- 99900546978701842
- Language
- English
- Resource Type
- Journal article