Arabidopsis P-protein filament formation requires both AtSEOR1 and AtSEOR2

James A Anstead; Daniel R Froelich; Michael Knoblauch; Gary A Thompson

doi:10.1093/pcp/pcs046

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Arabidopsis P-protein filament formation requires both AtSEOR1 and AtSEOR2

Journal article

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Arabidopsis P-protein filament formation requires both AtSEOR1 and AtSEOR2

James A Anstead, Daniel R Froelich, Michael Knoblauch and Gary A Thompson

Plant and cell physiology, Vol.53(6), pp.1033-1042

06/2012

DOI: https://doi.org/10.1093/pcp/pcs046

Handle:

https://hdl.handle.net/2376/106758

PMID: 22470058

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Abstract

DNA, Bacterial - metabolism

Genes, Plant

Herbivory - physiology

Open Reading Frames

Green Fluorescent Proteins - genetics

Structure-Activity Relationship

Aphids - pathogenicity

RNA, Messenger - metabolism

Genetic Complementation Test

Recombinant Fusion Proteins - metabolism

Arabidopsis Proteins - metabolism

Phloem - metabolism

Gene Expression Regulation, Plant

Phloem - genetics

Plasmids - genetics

Aphids - physiology

Host-Parasite Interactions

Green Fluorescent Proteins - metabolism

Arabidopsis Proteins - genetics

Plants, Genetically Modified - genetics

RNA, Messenger - genetics

Plasmids - metabolism

Arabidopsis - metabolism

Arabidopsis - genetics

Sequence Homology, Amino Acid

Animals

DNA, Bacterial - genetics

Plants, Genetically Modified - metabolism

Plants, Genetically Modified - parasitology

Recombinant Fusion Proteins - genetics

Arabidopsis - parasitology

Mutagenesis, Insertional

Antibodies, Monoclonal - metabolism

The structure-function relationship of proteinaceous filaments in sieve elements has long been a source of investigation in order to understand their role in the biology of the phloem. Two phloem filament proteins AtSEOR1 (At3g01680.1) and AtSEOR2 (At3g01670.1) in Arabidopsis have been identified that are required for filament formation. Immunolocalization experiments using a phloem filament-specific monoclonal antibody in the respective T-DNA insertion mutants provided an initial indication that both proteins are necessary to form phloem filaments. To investigate the relationship between these two proteins further, green fluorescent protein (GFP)-AtSEO fusion proteins were expressed in Columbia wild-type and T-DNA insertion mutants. Analysis of these mutants by confocal microscopy confirmed that phloem filaments could only be detected in the presence of both proteins, indicating that despite significant sequence homology the proteins are not functionally redundant. Individual phloem filament protein subunits of AtSEOR1 and AtSEOR2 were capable of forming homodimers, but not heterodimers in a yeast two-hybrid system. The absence of phloem filaments in phloem sieve elements did not result in gross alterations of plant phenotype or affect basal resistance to green peach aphid (Myzus persicae).

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Title: Arabidopsis P-protein filament formation requires both AtSEOR1 and AtSEOR2
Creators: James A Anstead - College of Agricultural Sciences, The Pennsylvania State University, University Park, PA 16802, USA. Jaa25@psu.edu
Daniel R Froelich
Michael Knoblauch
Gary A Thompson
Publication Details: Plant and cell physiology, Vol.53(6), pp.1033-1042
Academic Unit: Biological Sciences, School of
Publisher: Japan
Identifiers: 99900546981801842
Language: English
Resource Type: Journal article