Journal article
Arlee line of rainbow trout (Oncorhynchus mykiss) exhibits a low level of nonspecific cytotoxic cell activity
Developmental and comparative immunology, Vol.19(6), pp.497-505
1995
Handle:
https://hdl.handle.net/2376/106372
PMID: 8773199
Abstract
Nonspecific cytotoxic cell (NCC) activity was assessed in the peripheral blood of four isogenic lines of rainbow trout (Oncorhynchus
mykiss) which were derived by the chromosome set manipulation technique of androgenesis. In these fish, whose isogenicity was previously confirmed by multilocus DNA fingerprint analysis, NCC activity was studied by the release of
51Cr from YAC-1 targets. Two groups of trout (the homozygous Arlee 12 line and the heterozygous hybrid of the Arlee 63 and Arlee 12 lines) had significantly lower levels of NCC activity in peripheral blood than either outbred rainbow trout or other lines with Hot Creek or hybrid Arlee × Hot Creek ancestry. The low NCC activity in the Arlee line appears to be inherited as a recessive trait. Peripheral blood cells of the trout mediated lectin dependent cellular cytotoxicity (LDCC) with the addition of phytohemagglutirain to co-cultures of effector cells and YAC-1 cells. The low NCC activity in the peripheral blood of these fish is not due to a condition analogous to the NCC-deficient Chediak-Higashi syndrome of man or the beige mutation of mice.
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Details
- Title
- Arlee line of rainbow trout (Oncorhynchus mykiss) exhibits a low level of nonspecific cytotoxic cell activity
- Creators
- Sandra S Ristow - Department of Animal Sciences, Washington State University, Pullman, Washington 99164-6332, USALeslie D Grabowski - Department of Animal Sciences, Washington State University, Pullman, Washington 99164-6332, USAPaul A Wheeler - Department of Zoology, Washington State University, Pullman, Washington 99164-4236, USADavid J Prieur - Departrnent of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040, USAGary H Thorgaard - Department of Zoology, Washington State University, Pullman, Washington 99164-4236, USA
- Publication Details
- Developmental and comparative immunology, Vol.19(6), pp.497-505
- Academic Unit
- Biological Sciences, School of
- Publisher
- Elsevier Ltd
- Identifiers
- 99900546523501842
- Language
- English
- Resource Type
- Journal article