Journal article
Assays for chromatin remodeling during nucleotide excision repair in Saccharomyces cerevisiae
Methods (San Diego, Calif.), Vol.48(1), pp.19-22
2009
Handle:
https://hdl.handle.net/2376/114382
PMCID: PMC2692424
PMID: 19336254
Abstract
How DNA repair proteins interact with the dynamic structure of chromatin is an emerging question. Chromatin structure impedes the access of repair proteins to sites of DNA damage. Several recent studies have implicated chromatin remodeling complexes in DNA repair. In this report we summarize the methods we used to investigate chromatin remodeling during nucleotide excision repair (NER)
in vivo. We describe a procedure to analyze UV-induced chromatin remodeling at the silent mating-type locus
HML using isolated nuclei from UV-treated yeast cells. In addition, a method to capture transient protein–protein associations in chromatin is outlined. We have used the methods described here to demonstrate that the SWI/SNF chromatin remodeling complex is involved in chromatin rearrangement during NER.
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Details
- Title
- Assays for chromatin remodeling during nucleotide excision repair in Saccharomyces cerevisiae
- Creators
- Ling Zhang - Department of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine, 1011 NW 15th St., Miami, FL 33136, USAKristi Jones - Department of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine, 1011 NW 15th St., Miami, FL 33136, USAMichael J Smerdon - Biochemistry and Biophysics, School of Molecular Bioscience, Washington State University, Pullman, WA 99164-4660, USAFeng Gong - Department of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine, 1011 NW 15th St., Miami, FL 33136, USA
- Publication Details
- Methods (San Diego, Calif.), Vol.48(1), pp.19-22
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- Elsevier Inc
- Identifiers
- 99900547665801842
- Language
- English
- Resource Type
- Journal article