Journal article
Assessment of cross-protection to heterologous strains of Flavobacterium psychrophilum following vaccination with a live-attenuated coldwater disease immersion vaccine
Journal of fish diseases, Vol.42(1), pp.75-84
01/2019
Handle:
https://hdl.handle.net/2376/105168
PMID: 30370695
Abstract
Bacterial coldwater disease, caused by Flavobacterium psychrophilum, remains one of the most significant bacterial diseases of salmonids worldwide. A previously developed and reported live-attenuated immersion vaccine (F. psychrophilum; B.17-ILM) has been shown to confer significant protection to salmonids. To further characterize this vaccine, a series of experiments were carried out to determine the cross-protective efficacy of this B.17-ILM vaccine against 9 F. psychrophilum isolates (representing seven sequence types/three clonal complexes as determined by multilocus sequence typing) in comparison with a wild-type virulent strain, CSF-259-93. To assess protection, 28-day experimental challenges of rainbow trout (Oncorhynchus mykiss) fry were conducted following immersion vaccinations with the B.17-ILM vaccine. F. psychrophilum strains used in challenge trials were isolated from several fish species across the globe; however, all were found to be virulent in rainbow trout. The B.17-ILM vaccine provided significant protection against all strains, with relative percent survival values ranging from 51% to 72%. All vaccinated fish developed an adaptive immune response (as measured by F. psychrophilum-specific antibodies) that increased out to the time of challenge (8 weeks postimmunization). Previous studies have confirmed that antibody plays an important role in protection against F. psychrophilum challenge; therefore, specific antibodies to the B.17-ILM vaccine strain appear to contribute to the cross-protection observed to heterologous strain. The ability of such antibodies to bind to similar antigenic regions for all strains was confirmed by western blot analyses. Results presented here support the practical application of this live-attenuated vaccine, and suggest that it will be efficacious even in aquaculture operations affected by diverse strains of F. psychrophilum.
Metrics
12 Record Views
Details
- Title
- Assessment of cross-protection to heterologous strains of Flavobacterium psychrophilum following vaccination with a live-attenuated coldwater disease immersion vaccine
- Creators
- Jie Ma - Department of Fisheries and Wildlife Sciences and the Aquaculture Research Institute, College of Natural Resources, University of Idaho, Moscow, IdahoTimothy J Bruce - Department of Fisheries and Wildlife Sciences and the Aquaculture Research Institute, College of Natural Resources, University of Idaho, Moscow, IdahoPonnerassery S Sudheesh - Bronson Animal Disease Diagnostic Laboratory, Florida Department of Agriculture and Consumer Services, Kissimmee, FloridaChristopher Knupp - Department of Fisheries and Wildlife, College of Agriculture and Natural Resources, Michigan State University, East Lansing, MichiganThomas P Loch - Department of Pathobiology and Diagnostic Investigation, College of Veterinary Medicine, Michigan State University, East Lansing, MichiganMohamed Faisal - Department of Pathobiology and Diagnostic Investigation, College of Veterinary Medicine, Michigan State University, East Lansing, MichiganKenneth D Cain - Department of Fisheries and Wildlife Sciences and the Aquaculture Research Institute, College of Natural Resources, University of Idaho, Moscow, Idaho
- Publication Details
- Journal of fish diseases, Vol.42(1), pp.75-84
- Academic Unit
- Center for Reproductive Biology
- Publisher
- England
- Grant note
- RC105806 / USDA-NIFA Flavobacterial Diversity
- Identifiers
- 99900546733001842
- Language
- English
- Resource Type
- Journal article