Journal article
Association between mRNA abundance of functional sperm function proteins and fertility of Holstein bulls
Theriogenology, Vol.78(9), pp.2007-2019.e2
12/2012
Handle:
https://hdl.handle.net/2376/113026
PMID: 23040061
Abstract
Although the existence of a complex population of mRNA in sperm is well documented, its role has not been completely elucidated. The objective of this study was to determine the relationship of mRNA abundance of sperm specific proteins and sire conception rates (SCR; a fertility index) in Holstein bulls. Samples of sperm from a single collection from commercial Holstein bulls (N = 34) were used to evaluate relative mRNA expression of adenylate kinase (AK) 1, integrin beta (IB) 5, Doppel, nerve growth factor, tissue inhibitors of metalloproteinases (TIMP) 2, lactate dehydrogenase C 1, small nuclear ribonucleoprotein polypeptide N, outer dense fiber 2, and phospholipase C zeta (PLCz) 1 in sperm. With the exception of lactate dehydrogenase C 1 and outer dense fiber 2, the mRNA abundances of these proteins were greater (P < 0.05) for high fertility (> +2 to ≤ 4 SCR) bulls compared with average (≥ 2 to ≤ +2) and low fertility (> −2 to ≤ −4) bulls. Of all the multivariate regression models tested, a combination of AK1, IB5, TIMP2, small nuclear ribonucleoprotein polypeptide N, and PLCz1 accounted for 97.4% of the variance in SCR scores. In the absence of PLCz1, the combination of AK1, IB5, Doppel, nerve growth factor, TIMP, and small nuclear ribonucleoprotein polypeptide N accounted for 96.6% of the variance in SCR scores. In addition, immunocytochemistry confirmed that the sperm-specific protein markers evaluated in this study were present in sperm. In conclusion, frozen-thawed semen from bulls with higher AK1, IB5, TIMP, small nuclear ribonucleoprotein polypeptide N 2 and PLCz1 mRNA abundances in the sperm had greater correlations with sire fertility index and may possess greater probabilities of siring calves.
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Details
- Title
- Association between mRNA abundance of functional sperm function proteins and fertility of Holstein bulls
- Creators
- V Kasimanickam - Department of Veterinary Clinical Sciences, Washington State University, Pullman, Washington, USAR Kasimanickam - Department of Veterinary Clinical Sciences, Washington State University, Pullman, Washington, USAA Arangasamy - Department of Veterinary Clinical Sciences, Washington State University, Pullman, Washington, USAA Saberivand - Department of Veterinary Clinical Sciences, Washington State University, Pullman, Washington, USAJ.S Stevenson - Department of Animal Sciences and Industry, Kansas State University, Manhattan, Kansas, USAJ.P Kastelic - Agriculture and Agri-Food Canada, Lethbridge, Alberta, Canada
- Publication Details
- Theriogenology, Vol.78(9), pp.2007-2019.e2
- Academic Unit
- Center for Reproductive Biology; Veterinary Clinical Sciences, Department of
- Publisher
- Elsevier Inc
- Identifiers
- 99900547708601842
- Language
- English
- Resource Type
- Journal article