Journal article
Association of classical semen parameters, sperm DNA fragmentation index, lipid peroxidation and antioxidant enzymatic activity of semen in ram-lambs
Theriogenology, Vol.65(7), pp.1407-1421
2006
Handle:
https://hdl.handle.net/2376/106530
PMID: 16188307
Abstract
The objective was to determine relationships among classical semen characteristics, sperm chromatin structure assay (SCSA), lipid peroxidation and antioxidant enzymatic activity in ram-lamb semen. Fifty-seven ram-lambs were electroejaculated, and routine semen evaluation was conducted (as part of a breeding soundness evaluation). The percentage of sperm DNA fragmentation index (%DFI) and the percentage of sperm with abnormally high DNA stainability (HDS; immature spermatozoa) were determined by SCSA using the metachromatic properties of acridine orange. Semen was centrifuged at 800
×
g for 15
min to separate spermatozoa and seminal plasma and the aliquots were stored at −70
°C until analyzed. Lipid peroxidation, superoxide dismutase (SOD), and glutathione peroxidase (GPx) levels in seminal plasma and spermatozoa were measured by spectophotometric assays. The classical semen parameters were negatively related to lipid peroxidation and GPx activity in spermatozoa; motility and morphology were negatively related to %DFI (
P
<
0.05). Based on Kruskal–Wallis pair-wise comparison of median values among breeding soundness outcome groups, %DFI was lower in the satisfactory group compared to other groups (
P
<
0.05) and the lipid peroxidation and GPx activity in seminal plasma and spermatozoa were lower in satisfactory and questionable groups (
P
<
0.05). However, the SOD was lower in the unsatisfactory group (
P
<
0.05). In summary, classical semen parameters were negatively related to % DFI, lipid peroxidation and GPx activity in ram-lamb spermatozoa and seminal plasma. There were indications that SOD and GPx have crucial protective roles against the toxic effect of reactive oxygen species (ROS) in ram-lamb semen.
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Details
- Title
- Association of classical semen parameters, sperm DNA fragmentation index, lipid peroxidation and antioxidant enzymatic activity of semen in ram-lambs
- Creators
- Ramanathan Kasimanickam - Department of Large Animal Clinical Sciences, Virginia–Maryland Regional College of Veterinary Medicine, Blacksburg, VA 24061, USAKevin D Pelzer - Department of Large Animal Clinical Sciences, Virginia–Maryland Regional College of Veterinary Medicine, Blacksburg, VA 24061, USAVanmathy Kasimanickam - Department of Biomedical Sciences and Pathobiology, Virginia–Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USAWilliam S Swecker - Department of Large Animal Clinical Sciences, Virginia–Maryland Regional College of Veterinary Medicine, Blacksburg, VA 24061, USACraig D Thatcher - Department of Large Animal Clinical Sciences, Virginia–Maryland Regional College of Veterinary Medicine, Blacksburg, VA 24061, USA
- Publication Details
- Theriogenology, Vol.65(7), pp.1407-1421
- Academic Unit
- Center for Reproductive Biology; Veterinary Clinical Sciences, Department of
- Publisher
- Elsevier Inc
- Identifiers
- 99900546634101842
- Language
- English
- Resource Type
- Journal article