Association of human transferrin receptor with GABARAP

Frank Green; Thomas O’Hare; Aaron Blackwell; Caroline A Enns

doi:10.1016/S0014-5793(02)02655-8

Back

Association of human transferrin receptor with GABARAP

Journal article

Open access

Peer reviewed

Association of human transferrin receptor with GABARAP

Frank Green, Thomas O’Hare, Aaron Blackwell and Caroline A Enns

FEBS letters, Vol.518(1), pp.101-106

2002

DOI: https://doi.org/10.1016/S0014-5793(02)02655-8

Handle:

https://hdl.handle.net/2376/117395

PMID: 11997026

Files and links (1)

url

https://doi.org/10.1016/S0014-5793(02)02655-8View

Published (Version of record) Open

Abstract

Endocytic motif

γ-Aminobutyric acid type A receptor-associated protein

Transferrin receptor

Protein trafficking

A yeast two-hybrid screen identified a specific interaction between the cytoplasmic domain of transferrin receptor (TfR) and GABARAP, a 14 kDa protein that binds to the γ2 subunit of neuronal GABA A receptors. The specificity of the TfR–GABARAP interaction was demonstrated by in vitro binding assays with purified proteins and by co-immunoprecipitation of GABARAP with endogenous TfR from HeLa cell lysates. Replacement of the YTRF internalization motif with ATRA within the cytoplasmic domain of TfR reduced interaction with GABARAP in the yeast two-hybrid screen and in vitro binding assays. The intracellular location of GABARAP using chimeric GABARAP–GFP showed that the majority of GABARAP was located in perinuclear vesicles. Our results show that GABARAP plays a more general role outside the confines of neuronal cells and GABA A receptors.

Metrics

6 Record Views

See more details

Details

Title: Association of human transferrin receptor with GABARAP
Creators: Frank Green
Thomas O’Hare
Aaron Blackwell
Caroline A Enns
Publication Details: FEBS letters, Vol.518(1), pp.101-106
Publisher: Elsevier B.V
Identifiers: 99900548161901842
Language: English
Resource Type: Journal article