Journal article
Associations between bovine, human, and raw milk, and beef isolates of non-O157 Shiga toxigenic Escherichia coli within a restricted geographic area of the United States
Journal of food protection, Vol.71(5), pp.1023-1027
05/2008
Handle:
https://hdl.handle.net/2376/108895
PMID: 18522040
Abstract
A survey for Shiga toxigenic Escherichia coli in raw milk and beef was conducted within a defined geographic region of the United States. Prevalence rates based on detection of Shiga toxin gene (stx) were 36% for retail beef, 23% for beef carcasses, and 21% for raw milk samples, which were significantly higher than were Shiga toxigenic E. coli isolation rates of 7.5, 5.8, and 3.2%, respectively. Seasonal prevalence differences were significant for stx positivity among ground beef and milk samples. Distribution of stx subtypes among isolates varied according to sample type, with stx1 predominating in milk, stx2 on carcasses, and the combination of both stx1 and stx2 in beef. Ancillary virulence markers eae and ehx were evident in 23 and 15% of isolates, respectively. Pulsed-field gel electrophoresis demonstrated associations between food isolates and sympatric bovine fecal, and human clinical isolates. These data demonstrate that non-O157 Shiga toxigenic E. coli is present in the food chain in the Pacific Northwest, and its risk to health warrants critical assessment.
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Details
- Title
- Associations between bovine, human, and raw milk, and beef isolates of non-O157 Shiga toxigenic Escherichia coli within a restricted geographic area of the United States
- Creators
- R N Cobbold - Field Disease Investigation Unit, College of Veterinary Medicine, Washington State University, Pullman, Washington 99164-6610, USA. r.cobbold@uq.edu.auM A DavisD H RiceM SzymanskiP I TarrT E BesserD D Hancock
- Publication Details
- Journal of food protection, Vol.71(5), pp.1023-1027
- Academic Unit
- Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
- Publisher
- United States
- Grant note
- N01-AI-30055 / NIAID NIH HHS
- Identifiers
- 99900547037201842
- Language
- English
- Resource Type
- Journal article