Journal article
Atmospheric pressure matrix-assisted laser desorption/ionization with analysis by ion mobility time-of-flight mass spectrometry
Rapid communications in mass spectrometry, Vol.18(8), pp.882-888
2004
Handle:
https://hdl.handle.net/2376/116024
PMID: 15095357
Abstract
The use of an atmospheric pressure matrix-assisted laser desorption/ionization (AP-MALDI) source was employed with an atmospheric pressure ion mobility spectrometer (APIMS) and an orthogonal acceleration reflector time-of-flight mass spectrometer (TOFMS) to analyze dipeptide and biogenic amine mixtures from a liquid glycerol 2,5-dihydroxybenzoic acid (DHB) matrix. Improved sensitivities were obtained by the addition of a localized electrical (corona) discharge in conjunction with the AP-MALDI source. Enhanced sample ionization efficiency created by this combination provided an overall elevation in signal intensity of approximately 1.3 orders in magnitude. Combinations of three dipeptides (Gly-Lys, Ala-Lys, and Val-Lys) and nine biogenic amines (dopamine, serotonin, B-phenylethylamine, tyramine, octopamine, histamine, tryptamine, spermidine, and spermine) were resolved in less than 18 ms. In many cases, reduced mobility constants (K(o)) were determined for these analytes for the first time. Ion mobility drift times, flight times, arbitrary signal intensities, and collision-induced dissociation (CID) fragmentation product signatures are reported for each of the samples.
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Details
- Title
- Atmospheric pressure matrix-assisted laser desorption/ionization with analysis by ion mobility time-of-flight mass spectrometry
- Creators
- Wes E Steiner - Department of Chemistry, Washington State University, Pullman, WA 99164-4630, USABrian H ClowersWilliam A EnglishHerbert H Hill, Jr
- Publication Details
- Rapid communications in mass spectrometry, Vol.18(8), pp.882-888
- Academic Unit
- Chemistry, Department of
- Publisher
- England
- Identifiers
- 99900547927101842
- Language
- English
- Resource Type
- Journal article