Journal article
Attempts at validating a recombinant Flavobacterium psychrophilum gliding motility protein N as a vaccine candidate in rainbow trout, Oncorhynchus mykiss (Walbaum) against bacterial cold-water disease
FEMS microbiology letters, Vol.358(1), pp.14-20
09/01/2014
Handle:
https://hdl.handle.net/2376/104197
PMID: 25053267
Abstract
Abstract
The Flavobacterium psychrophilum gliding motility N (GldN) protein was investigated to determine its ability to elicit antibody responses and provide protective immunity in rainbow trout Oncorhynchus mykiss (Walbaum). GldN was PCR-amplified, cloned into pET102/D-TOPO, and expressed in Escherichia coli. Bacteria expressing recombinant GldN (rGldN) were formalin-inactivated and injected intraperitoneally (i.p.) into rainbow trout with Freund's complete adjuvant (FCA) in four separate studies that used two different immunization protocols followed by challenge evaluations. Fish injected with E. coli only in FCA served as the control. Antibody responses to F. psychrophilum whole-cell lysates measured by ELISA were low in all four studies. Protection against F. psychrophilum challenge was observed in the first study, but not in the three following studies. The discrepancies in results obtained in the later studies are unclear but may relate to formalin treatment of the antigen preparations. Overall, it appeared that rGldN delivered i.p. as a crude formalin-killed preparation is not a consistent vaccine candidate, and more work is required. Additionally, this study illustrates the importance of conducting multiple in vivo evaluations on potential vaccine(s) before any conclusions are drawn.
The Flavobacterium psychrophilum gliding motility protein (Gld N) was investigated to determine its ability to elicit antibody responses and provide protective immunity in rainbow trout Oncorhynchus mykiss.
The Flavobacterium psychrophilum gliding motility protein (Gld N) was investigated to determine its ability to elicit antibody responses and provide protective immunity in rainbow trout Oncorhynchus mykiss.
Metrics
4 Record Views
Details
- Title
- Attempts at validating a recombinant Flavobacterium psychrophilum gliding motility protein N as a vaccine candidate in rainbow trout, Oncorhynchus mykiss (Walbaum) against bacterial cold-water disease
- Creators
- Karen P Plant - Hagerman Fish Culture Experiment Station, University of Idaho, Hagerman, ID, USAScott E LaPatra - Research Division, Clear Springs Foods, Inc., Buhl, ID, USADouglas R Call - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, USAKenneth D Cain - Department of Fish and Wildlife Resources and The Aquaculture Research Institute, University of Idaho, Moscow, ID, USA
- Publication Details
- FEMS microbiology letters, Vol.358(1), pp.14-20
- Academic Unit
- Center for Reproductive Biology; Paul G. Allen School for Global Animal Health
- Publisher
- Blackwell Publishing Ltd; Oxford, UK
- Identifiers
- 99900546690901842
- Language
- English
- Resource Type
- Journal article