Journal article
Attenuation of DNA charge transport by compaction into a nucleosome core particle
Nucleic acids research, Vol.34(6), pp.1836-1846
2006
Handle:
https://hdl.handle.net/2376/114037
PMCID: PMC1428796
PMID: 16595797
Abstract
The nucleosome core particle (NCP) is the fundamental building block of chromatin which compacts approximately 146 bp of DNA around a core histone protein octamer. The effects of NCP packaging on long-range DNA charge transport reactions have not been adequately assessed to date. Here we study DNA hole transport reactions in a 157 bp DNA duplex (AQ-157TG) incorporating multiple repeats of the DNA TG-motif, a strong NCP positioning sequence and a covalently attached Anthraquinone photooxidant. Following a thorough biophysical characterization of the structure of AQ-157TG NCPs by Exonuclease III and hydroxyl radical footprinting, we compared the dynamics of DNA charge transport in ultraviolet-irradiated free and NCP-incorporated AQ-157TG. Compaction into a NCP changes the charge transport dynamics in AQ-157TG drastically. Not only is the overall yield of oxidative lesions decreased in the NCPs, but the preferred sites of oxidative damage change as well. This NCP-dependent attenuation of DNA charge transport is attributed to DNA-protein interactions involving the folded histone core since removal of the histone tails did not perturb the charge transport dynamics in AQ-157TG NCPs.
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Details
- Title
- Attenuation of DNA charge transport by compaction into a nucleosome core particle
- Creators
- Chad C Bjorklund - School of Molecular Biosciences, Fulmer Hall 275, Washington State University, Pullman, WA 99164-4660, USAWilliam B Davis
- Publication Details
- Nucleic acids research, Vol.34(6), pp.1836-1846
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- England
- Identifiers
- 99900547645501842
- Language
- English
- Resource Type
- Journal article