Journal article
Au@Pd Nanopopcorn and Aptamer Nanoflower Assisted Lateral Flow Strip for Thermal Detection of Exosomes
Analytical chemistry (Washington), Vol.91(21), pp.13986-13993
11/05/2019
PMID: 31486634
Abstract
Conventional lateral flow biosensing technologies face the dual formidable challenges of poor sensitivity and cumbersome quantitative devices. Here, we developed a Au@Pd nanopopcorn and aptamer nanoflower assisted lateral flow strip (ANAN-LFS) with a thermal signal output to improve detection sensitivity. Moreover, a smartphone-based thermal reader was designed and meticulously optimized to hand-held style, realizing the essential portability of this quantitative device. Experimental studies revealed that the synthesized Au@Pd nanopopcorns clearly red-shifted into the near-infrared region, thus resulting in a higher photothermal response than the standard gold nanoparticles. Aptamer nanoflowers enhanced the system's biorecognition ability significantly compared with single-stranded aptamers due to their functional spatial structure, thus resulting in an even greater improvement in the sensitivity of the ANAN-LFS. With exosomes as model targets, the limit of detection (LOD) was calculated to be 1.4 x 10(4) exosomes/mu L, which exhibited a 71-fold improved analytical performance. The feasibility of this system for detecting spiked biological samples at clinical concentrations was also confirmed. These results suggest that the proposed strategy of integrating a ANAN-LFS with a smartphone-based thermal reader has great potential as a powerful tool for bioanalytical applications, offering the combined unique advantages of high sensitivity and expedient portability.
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Details
- Title
- Au@Pd Nanopopcorn and Aptamer Nanoflower Assisted Lateral Flow Strip for Thermal Detection of Exosomes
- Creators
- Nan Cheng - China Agr Univ, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R ChinaYang Song - Washington State UniversityQiurong Shi - Washington State UniversityDan Du - Washington State UniversityDong Liu - Washington State UniversityYunbo Lu - China Agr Univ, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R ChinaWentao Xu - China Agr Univ, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R ChinaYuehe Lin - Washington State Univ, Sch Mech & Mat Engn, Pullman, WA 99164 USA
- Publication Details
- Analytical chemistry (Washington), Vol.91(21), pp.13986-13993
- Academic Unit
- School of Mechanical and Materials Engineering
- Publisher
- Amer Chemical Soc
- Number of pages
- 8
- Grant note
- 2018ZX08012-001 / National GMO Cultivation Major Project of New Varieties WSU Start-up fund BX20180364 / China National Postdoctoral Program for Innovative Talents 2018M640203 / China Postdoctoral Science Foundation
- Identifiers
- 99901227642801842
- Language
- English
- Resource Type
- Journal article