Journal article
Automated electric valve for electrokinetic separation in a networked microfluidic chip
Analytical chemistry (Washington), Vol.79(4), pp.1456-1465
02/15/2007
Handle:
https://hdl.handle.net/2376/110696
PMID: 17297944
Abstract
This paper describes an automated electric valve system designed to reduce dispersion and sample loss into a side channel when an electrokinetically mobilized concentration zone passes a T-junction in a networked microfluidic chip. One way to reduce dispersion is to control current streamlines since charged species are driven along them in the absence of electroosmotic flow. Computer simulations demonstrate that dispersion and sample loss can be reduced by applying a constant additional electric field in the side channel to straighten current streamlines in linear electrokinetic flow (zone electrophoresis). This additional electric field was provided by a pair of platinum microelectrodes integrated into the chip in the vicinity of the T-junction. Both simulations and experiments of this electric valve with constant valve voltages were shown to provide unsatisfactory valve performance during nonlinear electrophoresis (isotachophoresis). On the basis of these results, however, an automated electric valve system was developed with improved valve performance. Experiments conducted with this system showed decreased dispersion and increased reproducibility as protein zones isotachophoretically passed the T-junction. Simulations of the automated electric valve offer further support that the desired shape of current streamlines was maintained at the T-junction during isotachophoresis. Valve performance was evaluated at different valve currents based on statistical variance due to dispersion. With the automated control system, two integrated microelectrodes provide an effective way to manipulate current streamlines, thus acting as an electric valve for charged species in electrokinetic separations.
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Details
- Title
- Automated electric valve for electrokinetic separation in a networked microfluidic chip
- Creators
- Huanchun Cui - School of Chemical Engineering and Bioengineering and School of Mechanical and Materials Engineering, Washington State University, Pullman, Washington 99164, USAZheng HuangPrashanta DuttaCornelius F Ivory
- Publication Details
- Analytical chemistry (Washington), Vol.79(4), pp.1456-1465
- Academic Unit
- Chemical Engineering and Bioengineering, School of; Mechanical and Materials Engineering, School of
- Publisher
- United States
- Identifiers
- 99900547196901842
- Language
- English
- Resource Type
- Journal article