Journal article
Autophagy and metacaspase determine the mode of cell death in plants
The Journal of cell biology, Vol.203(6), pp.917-927
12/23/2013
Handle:
https://hdl.handle.net/2376/118376
PMCID: PMC3871426
PMID: 24344187
Abstract
Although animals eliminate apoptotic cells using macrophages, plants use cell corpses throughout development and disassemble cells in a cell-autonomous manner by vacuolar cell death. During vacuolar cell death, lytic vacuoles gradually engulf and digest the cytoplasmic content. On the other hand, acute stress triggers an alternative cell death, necrosis, which is characterized by mitochondrial dysfunction, early rupture of the plasma membrane, and disordered cell disassembly. How both types of cell death are regulated remains obscure. In this paper, we show that vacuolar death in the embryo suspensor of Norway spruce requires autophagy. In turn, activation of autophagy lies downstream of metacaspase mcII-Pa, a key protease essential for suspensor cell death. Genetic suppression of the metacaspase–autophagy pathway induced a switch from vacuolar to necrotic death, resulting in failure of suspensor differentiation and embryonic arrest. Our results establish metacaspase-dependent autophagy as a bona fide mechanism that is responsible for cell disassembly during vacuolar cell death and for inhibition of necrosis.
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Details
- Title
- Autophagy and metacaspase determine the mode of cell death in plants
- Creators
- Elena A Minina - Department of Plant Biology and Forest Genetics, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Center for Plant Biology, SE-75007 Uppsala, SwedenLada H Filonova - Department of Forest Products, Wood Science, Swedish University of Agricultural Sciences, SE-75007 Uppsala, SwedenKazutake Fukada - Department of Plant Biology and Forest Genetics, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Center for Plant Biology, SE-75007 Uppsala, SwedenEugene I Savenkov - Department of Plant Biology and Forest Genetics, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Center for Plant Biology, SE-75007 Uppsala, SwedenVladimir Gogvadze - Division of Toxicology, Institute of Environmental Medicine, Karolinska Institutet, SE-17177, Stockholm, SwedenDavid Clapham - Department of Plant Biology and Forest Genetics, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Center for Plant Biology, SE-75007 Uppsala, SwedenVictoria Sanchez-Vera - Department of Plant Biology and Forest Genetics, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Center for Plant Biology, SE-75007 Uppsala, Sweden, Departamento de Biologia Molecular y Bioquimica, Facultad de Ciencias, Universidad de Malaga, 290071 Malaga, SpainMaria F Suarez - Departamento de Biologia Molecular y Bioquimica, Facultad de Ciencias, Universidad de Malaga, 290071 Malaga, SpainBoris Zhivotovsky - Division of Toxicology, Institute of Environmental Medicine, Karolinska Institutet, SE-17177, Stockholm, SwedenGeoffrey Daniel - Department of Forest Products, Wood Science, Swedish University of Agricultural Sciences, SE-75007 Uppsala, SwedenAndrei Smertenko - The Integrative Cell Biology Laboratory, School of Biological and Biomedical Sciences, University of Durham, Durham DH1 3LE, England, UKPeter V Bozhkov - Department of Plant Biology and Forest Genetics, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Center for Plant Biology, SE-75007 Uppsala, Sweden
- Publication Details
- The Journal of cell biology, Vol.203(6), pp.917-927
- Academic Unit
- Biological Chemistry, Institute of
- Identifiers
- 99900548585201842
- Language
- English
- Resource Type
- Journal article