Autophagy proteins play cytoprotective and cytocidal roles in leucine starvation-induced cell death in Saccharomyces cerevisiae

Slawomir A Dziedzic; Allan B Caplan

doi:10.4161/auto.19314

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Autophagy proteins play cytoprotective and cytocidal roles in leucine starvation-induced cell death in Saccharomyces cerevisiae

Journal article

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Autophagy proteins play cytoprotective and cytocidal roles in leucine starvation-induced cell death in Saccharomyces cerevisiae

Slawomir A Dziedzic and Allan B Caplan

Autophagy, Vol.8(5), pp.731-738

05/01/2012

DOI: https://doi.org/10.4161/auto.19314

Handle:

https://hdl.handle.net/2376/102200

PMCID: PMC3378417

PMID: 22361650

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Abstract

Green Fluorescent Proteins - metabolism

Leucine - metabolism

Nitrogen - metabolism

Microbial Viability - drug effects

Apoptosis - drug effects

Leucine - deficiency

Nitrogen - deficiency

Saccharomyces cerevisiae - drug effects

Mutation - genetics

Sirolimus - pharmacology

Autophagy - drug effects

Saccharomyces cerevisiae - cytology

Saccharomyces cerevisiae - metabolism

Cytoprotection - drug effects

Saccharomyces cerevisiae Proteins - metabolism

Autophagy is essential for prolonging yeast survival during nutrient deprivation; however, this report shows that some autophagy proteins may also be accelerating population death in those conditions. While leucine starvation caused YCA1-mediated apoptosis characterized by increased annexin V staining, nitrogen deprivation triggered necrotic death characterized by increased propidium iodide uptake. Although a Δatg8 strain died faster than its parental strain during nitrogen starvation, this mutant died slower than its parent during leucine starvation. Conversely, a Δatg11 strain died slower than its parent during nitrogen starvation, but faster during leucine starvation. Curiously, although GFP-Atg8 complemented the Δatg8 mutation, this protein made ATG8 cells more sensitive to nitrogen starvation, and less sensitive to leucine starvation. These results were difficult to explain if autophagy only extended life but could be an indication that a second form of autophagy could concurrently facilitate either apoptotic or necrotic cell death.

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Title: Autophagy proteins play cytoprotective and cytocidal roles in leucine starvation-induced cell death in Saccharomyces cerevisiae
Creators: Slawomir A Dziedzic - Department of Plant, Soil, and Entomological Sciences and Program for Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, ID USA
Allan B Caplan
Publication Details: Autophagy, Vol.8(5), pp.731-738
Academic Unit: UNKNOWN
Publisher: United States
Grant note: P20 RR016448 / NCRR NIH HHS
Identifiers: 99900546615501842
Language: English
Resource Type: Journal article