Journal article
Axenic growth of Coxiella burnetii
Advances in experimental medicine and biology, Vol.984, pp.215-229
2012
Handle:
https://hdl.handle.net/2376/101178
PMID: 22711634
Abstract
Early metabolic studies of C. burnetii showed minimal metabolic activity of axenic (host cell-free) organisms in buffers adjusted to neutral pH. However, our understanding of the organism's physiology was greatly improved upon the discovery that C. burnetii requires an acidic pH for metabolic activation. Indeed, information gained from acid activation studies coupled with contemporary analyses using transcription microarrays, metabolic pathway reconstruction and metabolite typing, led to an axenic culture system that supports robust growth of C. burnetii. While axenic culture of C. burnetii can present some technical challenges, the technique is currently facilitating new lines of investigation and development of genetic tools. Axenic cultivation of C. burnetii should also prove useful in clinical settings.
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Details
- Title
- Axenic growth of Coxiella burnetii
- Creators
- Anders Omsland - Coxiella Pathogenesis Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 South 4th Street, Hamilton, MT 59840, USA. omslanda@niaid.nih.gov
- Publication Details
- Advances in experimental medicine and biology, Vol.984, pp.215-229
- Academic Unit
- Paul G. Allen School for Global Animal Health
- Publisher
- United States
- Grant note
- Intramural NIH HHS
- Identifiers
- 99900546644001842
- Language
- English
- Resource Type
- Journal article