Journal article
Babesia bigemina: Quantitation of infection in nymphal and adult Boophilus microplus using a DNA probe
Experimental parasitology, Vol.74(1), pp.117-126
1992
Handle:
https://hdl.handle.net/2376/110832
PMID: 1730269
Abstract
Candidates for a subunit vaccine against bovine babesiosis include surface proteins of infective forms found in the salivary glands of tick vectors. However, low numbers of infective forms are present within ticks and hinder analysis of this stage. To solve this problem, conditions which yield high numbers of infective forms were investigated with the use of a
Babesia bigemina-specific DNA probe. DNA from progeny of female
Boophilus microplus infected with
B. bigemina was hybridized to probe DNA to detect and quantitate infection. There was no difference in the prevalence of infection in progeny of three strains of
Bo. microplus. However, within a strain, prevalence could be increased to 30% by combining selection of progeny from heavily (3+) infected female ticks and selection of eggs laid 120 hr postengorgement. Quantitation of infective forms within pooled salivary gland preparations of 10 infected nymphal and adult
Bo. microplus demonstrated that Day 9 and 10 nymphal ticks contained the highest numbers of parasites and represented approximately 10
6 infective forms. This number of infective forms is suitable for isolation and further characterization.
Metrics
8 Record Views
Details
- Title
- Babesia bigemina: Quantitation of infection in nymphal and adult Boophilus microplus using a DNA probe
- Creators
- Jennifer L Hodgson - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, U.S.ADavid Stiller - Animal Disease Research Unit, USDA-ARS, Moscow, Idaho, U.S.ADouglas P Jasmer - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, U.S.AGerald M Buening - Department of Veterinary Microbiology, University of Missouri-Columbia, Columbia, Missouri, U.S.AGale G Wagner - Center for Tropical Animal Health, College of Veterinary Medicine, Texas A & M University, College Station, Texas, U.S.ATravis C McGuire - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, U.S.A
- Publication Details
- Experimental parasitology, Vol.74(1), pp.117-126
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- Elsevier Inc
- Identifiers
- 99900547047301842
- Language
- English
- Resource Type
- Journal article