Journal article
Base excision repair of N-methylpurines in a yeast minichromosome. Effects of transcription, dna sequence, and nucleosome positioning
The Journal of biological chemistry, Vol.274(18), pp.12201-12204
04/30/1999
Handle:
https://hdl.handle.net/2376/110497
PMID: 10212183
Abstract
Base excision repair of dimethyl sulfate induced N-methylpurines (NMPs) was measured in a yeast minichromosome that has a galactose-inducible GAL1:URA3 fusion gene, a constitutively expressed HIS3 gene, and varied regions of chromatin structure. Removal rates of NMPs varied dramatically (>20-fold) at different sites along three selected fragments encompassing a total length of 1775 base pairs. Repair of NMPs was not coupled to transcription, because the transcribed strands of HIS3 and induced GAL1:URA3 were not repaired faster than the nontranscribed strands. However, the repair rate of NMPs was significantly affected by the nearest neighbor nucleotides. Slow repair occurred at NMPs between purines, especially guanines, whereas fast repair occurred at NMPs between pyrimidines. NMPs between a purine and pyrimidine were repaired at moderate rates. Moreover, a rough correlation between nucleosome positions and repair rates exists in some but not all regions that were analyzed.
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Details
- Title
- Base excision repair of N-methylpurines in a yeast minichromosome. Effects of transcription, dna sequence, and nucleosome positioning
- Creators
- S Li - Department of Biochemistry and Biophysics, Washington State University, Pullman, Washington 99164-4660, USAM J Smerdon
- Publication Details
- The Journal of biological chemistry, Vol.274(18), pp.12201-12204
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- United States
- Grant note
- ES04106 / NIEHS NIH HHS
- Identifiers
- 99900547273901842
- Language
- English
- Resource Type
- Journal article