Journal article
Bcl-2 in endothelial cells is increased by vitamin E and α-lipoic acid supplementation but not exercise training
Journal of molecular and cellular cardiology, Vol.38(3), pp.445-451
2005
Handle:
https://hdl.handle.net/2376/116091
PMID: 15733904
Abstract
Atherosclerotic plaque contains apoptotic endothelial cells with oxidative stress implicated in this process. Vitamin E and α-lipoic acid are a potent antioxidant combination with the potential to prevent endothelial apoptosis. Regular exercise is known to increase myocardial protection, however, little research has investigated the effects of exercise on the endothelium. The purpose of these studies was to investigate the effects of antioxidant supplementation and/or exercise training on proteins that regulate apoptosis in endothelial cells. Male rats received a control or antioxidant-supplemented diet (vitamin E and α-lipoic acid) and were assigned to sedentary or exercise-trained groups for 14 weeks. Left ventricular endothelial cells (LVECs) were isolated and levels of the anti-apoptotic protein Bcl-2 and the pro-apoptotic protein Bax were measured. Antioxidant supplementation caused a fourfold increase in Bcl-2 (
P < 0.05) with no change in Bax (
P > 0.05). Bcl-2:Bax was increased sixfold with antioxidant supplementation compared to non-supplemented animals (
P < 0.05). Exercise training had no significant effect on Bcl-2, Bax or Bcl-2:Bax either alone or combined with antioxidant supplementation (
P > 0.05) compared to non-supplemented animals. However, Bax was significantly lower (
P < 0.05) in the supplemented trained group compared to non-supplemented trained animals. Cultured bovine endothelial cells incubated for 24 h with vitamin E and/or α-lipoic acid showed the combination of the two antioxidants increased Bcl-2 to a greater extent than cells incubated with the vehicle alone. In summary, vitamin E and α-lipoic acid increase endothelial cell Bcl-2, which may provide increased protection against apoptosis.
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Details
- Title
- Bcl-2 in endothelial cells is increased by vitamin E and α-lipoic acid supplementation but not exercise training
- Creators
- Susan A Marsh - School of Human Movement Studies, The University of Queensland, Q 4072 Brisbane, AustraliaPaul B Laursen - School of Human Movement Studies, The University of Queensland, Q 4072 Brisbane, AustraliaBetty K Pat - School of Medicine, The University of Queensland, 4072 Brisbane, AustraliaGlenda C Gobe - School of Medicine, The University of Queensland, 4072 Brisbane, AustraliaJeff S Coombes - School of Human Movement Studies, The University of Queensland, Q 4072 Brisbane, Australia
- Publication Details
- Journal of molecular and cellular cardiology, Vol.38(3), pp.445-451
- Academic Unit
- Pharmaceutical Sciences, Department of
- Publisher
- Elsevier Ltd
- Identifiers
- 99900547936201842
- Language
- English
- Resource Type
- Journal article