Journal article
Beta-glucuronidase as reporter gene: advantages and limitations
Methods in molecular biology (Clifton, N.J.), Vol.323, pp.263-273
2006
Handle:
https://hdl.handle.net/2376/109599
PMID: 16739584
Abstract
The beta-glucuronidase (GUS) gene is used extensively in plant biology studies; this analysis summarizes its advantages and limitations. With the advances in genomic sequencing and computational analyses (including bioinformatics), its application in the study of plant gene expression is now an integral component of modern day plant science. This chapter focuses on the detailed challenges of carrying out GUS studies for both qualitative and quantitative analyses, including the increasing employment of GUS from Bacillus strains, rather than E. coli; the Bacillus GUS genes encode proteins with enhanced properties, such as both increased thermostability and stability in the presence of crosslinking fixatives.
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Details
- Title
- Beta-glucuronidase as reporter gene: advantages and limitations
- Creators
- Kye-Won Kim - Institute of Biological Chemistry, Washington State University, Pullman, USAVincent R FranceschiLaurence B DavinNorman G Lewis
- Publication Details
- Methods in molecular biology (Clifton, N.J.), Vol.323, pp.263-273
- Academic Unit
- Biological Chemistry, Institute of
- Publisher
- United States
- Identifiers
- 99900547433001842
- Language
- English
- Resource Type
- Journal article