Journal article
Biased Immunoglobulin G1 Isotype Responses Induced in Cattle with DNA Expressing msp1a of Anaplasma marginale
Infection and immunity, Vol.67(7), pp.3481-3487
07/1999
Handle:
https://hdl.handle.net/2376/101679
PMCID: PMC116534
PMID: 10377129
Abstract
Immunization with the native major surface protein 1 (MSP1) (a heterodimer containing disulfide and noncovalently bonded polypeptides designated MSP1a and MSP1b) of the erythrocytic stage of
Anaplasma marginale
conferred protection against homologous challenge (G. H. Palmer, A. F. Barbet, W. C. Davis, and T. C. McGuire, Science 231:1299–1302, 1986). The MSP1a polypeptide possesses a conserved neutralization-sensitive epitope. In the present study, the immune response to DNA-mediated immunization using
msp
1a was studied. The plasmid pVCL/MSP1a, which encodes the complete
msp
1a gene of
A. marginale
under the control of human cytomegalovirus immediate-early enhancer/promoter and intron A, was constructed. The immune responses elicited by immunization with pVCL/MSP1a into cardiotoxin-induced regenerating muscle were evaluated in mice and cattle. Antibody reactive with native MSP1a was detected in pooled sera of immunized BALB/c mice 3 weeks following primary immunization. Two calves seronegative for
A. marginale
were immunized four times, at weeks 0, 3, 7, and 13, with pVCL/MSP1a. By 8 weeks, both calves responded to MSP1a with an antibody titer of 1:100, which peaked at 1:1,600 and 1:800 by 16 weeks after the initial immunization. Interestingly, immunoblotting with anti-immunoglobulin G1 (anti-IgG1) and anti-IgG2 specific monoclonal antibodies revealed a restricted IgG1 anti-MSP1a response in both animals. T-lymphocyte lines, established after the fourth immunization, proliferated specifically against
A. marginale
homogenate and purified MSP1 in a dose-dependent manner. These data provide a basis for an immunization strategy to direct bovine immune responses by using DNA vaccine vectors containing single or multiple genes encoding major surface proteins of
A. marginale
.
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Details
- Title
- Biased Immunoglobulin G1 Isotype Responses Induced in Cattle with DNA Expressing msp1a of Anaplasma marginale
- Creators
- Appudurai Arulkanthan - Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, College of Veterinary MedicineWendy C Brown - Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, College of Veterinary MedicineTravis C McGuire - Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, College of Veterinary MedicineDonald P Knowles - Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine
- Publication Details
- Infection and immunity, Vol.67(7), pp.3481-3487
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- American Society for Microbiology
- Identifiers
- 99900546567201842
- Language
- English
- Resource Type
- Journal article