Journal article
Bibersteinia trehalosi Inhibits the Growth of Mannheimia haemolytica by a Proximity-Dependent Mechanism
Applied and environmental microbiology, Vol.76(4), pp.1008-1013
02/2010
Handle:
https://hdl.handle.net/2376/107343
PMCID: PMC2820941
PMID: 20038698
Abstract
Mannheimia
(
Pasteurella
)
haemolytica
is the only pathogen that consistently causes severe bronchopneumonia and rapid death of bighorn sheep (BHS;
Ovis canadensis
) under experimental conditions. Paradoxically,
Bibersteinia
(
Pasteurella
)
trehalosi
and
Pasteurella multocida
have been isolated from BHS pneumonic lungs much more frequently than
M. haemolytica
. These observations suggest that there may be an interaction between these bacteria, and we hypothesized that
B. trehalosi
overgrows or otherwise inhibits the growth of
M. haemolytica
. Growth curves (monoculture) demonstrated that
B. trehalosi
has a shorter doubling time (∼10 min versus ∼27 min) and consistently achieves 3-log higher cell density (CFU/ml) compared to
M. haemolytica
. During coculture
M. haemolytica
growth was inhibited when
B. trehalosi
entered stationary phase (6 h) resulting in a final cell density for
M. haemolytica
that was 6 to 9 logs lower than expected with growth in the absence of
B. trehalosi
. Coculture supernatant failed to inhibit
M. haemolytica
growth on agar or in broth, indicating no obvious involvement of lytic phages, bacteriocins, or quorum-sensing systems. This observation was confirmed by limited growth inhibition of
M. haemolytica
when both pathogens were cultured in the same media but separated by a filter (0.4-μm pore size) that limited contact between the two bacterial populations. There was significant growth inhibition of
M. haemolytica
when the populations were separated by membranes with a pore size of 8 μm that allowed free contact. These observations demonstrate that
B. trehalosi
can both outgrow and inhibit
M. haemolytica
growth with the latter related to a proximity- or contact-dependent mechanism.
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Details
- Title
- Bibersteinia trehalosi Inhibits the Growth of Mannheimia haemolytica by a Proximity-Dependent Mechanism
- Creators
- Rohana P Dassanayake - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040Douglas R Call - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040Ashish A Sawant - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040N. Carol Casavant - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040Glen C Weiser - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040Donald P Knowles - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040Subramaniam Srikumaran - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040
- Publication Details
- Applied and environmental microbiology, Vol.76(4), pp.1008-1013
- Academic Unit
- Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
- Publisher
- American Society for Microbiology (ASM)
- Identifiers
- 99900547632601842
- Language
- English
- Resource Type
- Journal article