Journal article
Bighorn sheep beta2-integrin LFA-1 serves as a receptor for Mannheimia haemolytica leukotoxin
Journal of wildlife diseases, Vol.44(3), pp.743-747
07/2008
Handle:
https://hdl.handle.net/2376/113614
PMID: 18689665
Abstract
Mannheimia haemolytica is an important cause of pneumonia in bighorn sheep (BHS; Ovis canadensis). Leukotoxin (Lkt), the primary virulence determinant of M. haemolytica, induces cytolysis of all subsets of leukocytes. Previously, we have shown that CD18, the beta subunit of beta2-integrins, mediates Lkt-induced cytolysis. However, it is not clear whether CD18 of all three beta2-integrins, LFA-1, Mac-1, and CR4, mediates Lkt-induced cytolysis. The objective of this study was to determine whether BHS LFA-1 (CD11a/CD18) serves as a receptor for Lkt. Plasmids encoding cDNA for BHS CD11a and CD18 were cotransfected into Lkt-resistant HEK-293 cells. Flow cytometric analysis of transfectants confirmed cell surface expression of BHS LFA- 1, Lkt-LFA-1 binding and Lkt-induced intra-cellular calcium elevation. More importantly, the transfectants were efficiently lysed by Lkt in a concentration-dependent manner. Collectively, these results indicate that BHS LFA-1 serves as a functional receptor for M. haemolytica Lkt.
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Details
- Title
- Bighorn sheep beta2-integrin LFA-1 serves as a receptor for Mannheimia haemolytica leukotoxin
- Creators
- Rohana P Dassanayake - Department of Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040, USAWeiguo LiuWilliam C DavisWilliam J ForeytSubramaniam Srikumaran
- Publication Details
- Journal of wildlife diseases, Vol.44(3), pp.743-747
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- United States
- Identifiers
- 99900548355201842
- Language
- English
- Resource Type
- Journal article