Journal article
Binding of fibronectin by Trichomonas vaginalis is influenced by iron and calcium
Microbial pathogenesis, Vol.31(3), pp.131-144
09/2001
Handle:
https://hdl.handle.net/2376/109144
PMID: 11500098
Abstract
We have reported that Trichomonas vaginalis, the causative agent of the most common, non-viral sexually transmitted disease, bound to cover slips coated with fibronectin (FN) (Crouch & Alderete, Microbiol 1999145 : 2835–43). In this study, we extend that observation by showing that FN binding is specific, and we present data on the requirements of FN binding by T. vaginalis. Immunofluorescence and immuno-gold labelling readily detected FN throughout the trichomonal surface. Parasites bound to125I-labelled FN in a time- and concentration-dependent fashion. In the absence of protease inhibitor, iodinated FN was released from the trichomonad surface. Unlabelled FN specifically competed for binding in a concentration-dependent fashion with the125I-labelled FN. Interestingly, the amount of FN bound byT. vaginalis organisms was dependent on iron. High-iron-grown trichomonads acquired lower numbers of molecules but with 10-fold higher affinity than low-iron-grown organisms. Further, we show that for iron-replete organisms, calcium (Ca2+) at physiological levels increased amounts of bound FN. The increase in binding was rapid, occurring within 5 min of Ca2+addition, and required de novo protein synthesis. Co-incubation of live parasites with Ca2+in the presence of FN was necessary to increase the amount of FN bound. Treatment of trichomonads with okadaic acid, but not other phosphatase inhibitors, resulted in a 50% decrease in binding of FN, regardless of the presence of Ca2+, suggesting a role for phosphatase in FN association. These results indicate that depending on the iron status of T. vaginalis organisms in vivo, Ca2+may influence trichomonad recognition and binding to FN during host parasitism.
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Details
- Title
- Binding of fibronectin by Trichomonas vaginalis is influenced by iron and calcium
- Creators
- M.-L Crouch - Department of Microbiology, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX, 78284-7758, U.S.AMarlene Benchimol - Rio de Janeiro Universidade Santa Ursula, Rio de Janeiro, BrasilJ.F Alderete - Department of Microbiology, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX, 78284-7758, U.S.A
- Publication Details
- Microbial pathogenesis, Vol.31(3), pp.131-144
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- Elsevier Ltd
- Identifiers
- 99900547138101842
- Language
- English
- Resource Type
- Journal article