Binding of human angiogenin inhibits actin polymerization

Mikhail G Pyatibratov; Dmitri Tolkatchev; Josee Plamondon; Ping Xu; Feng Ni; Alla S Kostyukova

doi:10.1016/j.abb.2009.12.024

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Binding of human angiogenin inhibits actin polymerization

Journal article

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Peer reviewed

Binding of human angiogenin inhibits actin polymerization

Mikhail G Pyatibratov, Dmitri Tolkatchev, Josee Plamondon, Ping Xu, Feng Ni and Alla S Kostyukova

Archives of biochemistry and biophysics, Vol.495(1), pp.74-81

03/01/2010

DOI: https://doi.org/10.1016/j.abb.2009.12.024

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https://hdl.handle.net/2376/113019

PMCID: PMC2831474

PMID: 20045391

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Abstract

Actin Cytoskeleton - metabolism

Animals

Ribonuclease, Pancreatic - metabolism

Chickens

Humans

Actins - metabolism

Nuclear Magnetic Resonance, Biomolecular

Protein Binding

Angiogenin is a potent inducer of angiogenesis, a process of blood vessel formation. It interacts with endothelial and other cells and elicits a wide range of cellular responses including migration, proliferation, and tube formation. One important target of angiogenin is endothelial cell-surface actin and their interaction might be one of essential steps in angiogenin-induced neovascularization. Based on earlier indications that angiogenin promotes actin polymerization, we studied the binding interactions between angiogenin and actin in a wide range of conditions. We showed that at subphysiological KCl concentrations, angiogenin does not promote, but instead inhibits polymerization by sequestering G-actin. At low KCl concentrations angiogenin induces formation of unstructured aggregates, which, as shown by NMR, may be caused by angiogenin's propensity to form oligomers. Binding of angiogenin to preformed F-actin does not cause depolymerization of actin filaments though it causes their stiffening. Binding of tropomyosin and angiogenin to F-actin is not competitive at concentrations sufficient for saturation of actin filaments. These observations suggest that angiogenin may cause changes in the cell cytoskeleton by inhibiting polymerization of G-actin and changing the physical properties of F-actin.

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Title: Binding of human angiogenin inhibits actin polymerization
Creators: Mikhail G Pyatibratov - Institute of Protein Research, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russia. bratov@vega.protres.ru
Dmitri Tolkatchev
Josee Plamondon
Ping Xu
Feng Ni
Alla S Kostyukova
Publication Details: Archives of biochemistry and biophysics, Vol.495(1), pp.74-81
Academic Unit: Chemical Engineering and Bioengineering, School of
Publisher: United States
Grant note: R01 GM081688 / NIGMS NIH HHS GM081688 / NIGMS NIH HHS R01 GM081688-02 / NIGMS NIH HHS
Identifiers: 99900547989401842
Language: English
Resource Type: Journal article