Journal article
Biochemical characterization of a new β-glucosidase (Cel3E) from Penicillium piceum and its application in boosting lignocelluloses bioconversion and forming disaccharide inducers: New insights into the role of β-glucosidase
Process biochemistry (1991), Vol.49(5), pp.768-774
05/2014
Handle:
https://hdl.handle.net/2376/118001
Abstract
•This is the first paper on the characteristics and functions of Cel3E.•The Km of PpCel3E was the lowest for pNPG ever reported fungal β-glucosidase.•PpCel3E had a high transglycosylation activity to form disaccharide derivatives.•PpCel3E plays an important role in forming inducer and amplifying inducer signal.•PpCel3E with low dosage could enhance cellulase efficiency by 20–27%.
Fungal genome sequencing has revealed the presence of multiple putative β-glucosidases; however, information regarding these new β-glucosidases is limited. A new β-glucosidase from Penicillium piceum, designated as PpCel3E, was first isolated and characterized. Using p-nitrophenyl-β-d-glucoside as substrate, PpCel3E showed the lowest Km among the β-glucosidases among all fungi studied. Moreover, PpCel3E exhibited a high transglycosylation activity of 1100mg gentiobiose/mg and 142mg sophorose/mg using glucose as the donor. PpCel3E is a novel bifunctional glycoside hydrolase with both β-glucosidase and β-xylosidase activity. Our results show that PpCel3E plays an important role in forming soluble cellulose inducer compounds, as well as in amplifying weak cellulase inducer signal and hemicellulase synthesis via its high transglycosylation activity. Supplementing PpCel3E at low concentrations (40μg/g substrate) increased the saccharification efficiency of different cellulases by 20% to 27% by removing multiple inhibitors.
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Details
- Title
- Biochemical characterization of a new β-glucosidase (Cel3E) from Penicillium piceum and its application in boosting lignocelluloses bioconversion and forming disaccharide inducers: New insights into the role of β-glucosidase
- Creators
- Le Gao - Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, ChinaFeng Gao - Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, ChinaXukai Jiang - State Key Laboratory of Microbial Technology, Shandong University, Jinan, ChinaCan Zhang - Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, ChinaDongyuan Zhang - Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, ChinaLushan Wang - State Key Laboratory of Microbial Technology, Shandong University, Jinan, ChinaGaihong Wu - Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, ChinaShulin Chen - Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China
- Publication Details
- Process biochemistry (1991), Vol.49(5), pp.768-774
- Publisher
- Elsevier Ltd
- Identifiers
- 99900582323601842
- Language
- English
- Resource Type
- Journal article