Journal article
Biofilm shows spatially stratified metabolic responses to contaminant exposure
Environmental microbiology, Vol.14(11), pp.2901-2910
11/2012
Handle:
https://hdl.handle.net/2376/115045
PMCID: PMC3480979
PMID: 22925136
Abstract
Biofilms are core to a range of biological processes, including the bioremediation of environmental contaminants. Within a biofilm population, cells with diverse genotypes and phenotypes coexist, suggesting that distinct metabolic pathways may be expressed based on the local environmental conditions in a biofilm. However, metabolic responses to local environmental conditions in a metabolically active biofilm interacting with environmental contaminants have never been quantitatively elucidated. In this study, we monitored the spatiotemporal metabolic responses of metabolically active
Shewanella oneidensis
MR-1 biofilms to U(VI) (uranyl, UO
2
2+
) and Cr(VI) (chromate, CrO
4
2−
) using noninvasive nuclear magnetic resonance imaging (MRI) and spectroscopy (MRS) approaches to obtain insights into adaptation in biofilms during biofilm-contaminant interactions. While overall biomass distribution was not significantly altered upon exposure to U(VI) or Cr(VI), MRI and spatial mapping of the diffusion revealed localized changes in the water diffusion coefficients in the biofilms, suggesting significant contaminant-induced changes in structural or hydrodynamic properties during bioremediation. Finally, we quantitatively demonstrated that the metabolic responses of biofilms to contaminant exposure are spatially stratified, implying that adaptation in biofilms is custom-developed based on local microenvironments.
Metrics
6 Record Views
Details
- Title
- Biofilm shows spatially stratified metabolic responses to contaminant exposure
- Creators
- Bin Cao - Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USAPaul D Majors - Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USABulbul Ahmed - The Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA, USARyan S Renslow - The Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA, USACrystal P Silvia - Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USALiang Shi - Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USAStaffan Kjelleberg - Singapore Centre on Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, SingaporeJim K Fredrickson - Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USAHaluk Beyenal - The Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA, USA
- Publication Details
- Environmental microbiology, Vol.14(11), pp.2901-2910
- Academic Unit
- Chemical Engineering and Bioengineering, School of
- Grant note
- R01 ES017070 || ES / National Institute of Environmental Health Sciences : NIEHS
- Identifiers
- 99900547414201842
- Language
- English
- Resource Type
- Journal article