Bisphenol A effects on the growing mouse oocyte are influenced by diet

Ailene Muhlhauser; Martha Susiarjo; Carmen Rubio; Jodi Griswold; Galen Gorence; Terry Hassold; Patricia A Hunt

doi:10.1095/biolreprod.108.074815

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Bisphenol A effects on the growing mouse oocyte are influenced by diet

Journal article

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Bisphenol A effects on the growing mouse oocyte are influenced by diet

Ailene Muhlhauser, Martha Susiarjo, Carmen Rubio, Jodi Griswold, Galen Gorence, Terry Hassold and Patricia A Hunt

Biology of reproduction, Vol.80(5), pp.1066-1071

05/2009

DOI: https://doi.org/10.1095/biolreprod.108.074815

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https://hdl.handle.net/2376/105839

PMID: 19164168

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Abstract

Oocytes - ultrastructure

Oocytes - growth & development

Benzhydryl Compounds

Phenols - toxicity

Mice, Inbred C57BL

Aneuploidy

Endocrine Disruptors - administration & dosage

Endocrine Disruptors - toxicity

Meiosis - genetics

Meiosis - drug effects

Animals

Estrogens, Non-Steroidal - administration & dosage

Animal Feed - analysis

Diet

Estrogens, Non-Steroidal - toxicity

Oocytes - drug effects

Female

Mice

Phytoestrogens - administration & dosage

Isoflavones - administration & dosage

Growing evidence suggests that exposure to bisphenol A (BPA) has the ability to disrupt several different stages of oocyte development. To date, most attention has focused on the effects of BPA on the periovulatory oocyte, and considerable variation is evident in the results of these studies. In our own laboratory, variation in the results of BPA studies conducted at different times appeared to correlate with changes in mill dates of animal feed. This observation, coupled with reports by others that dietary estrogens in feed are a confounding variable in studies of endocrine-disrupting chemicals, prompted us to evaluate the effect of diet on the results of BPA studies of the periovulatory oocyte. Genetically identical females were placed on a high- or low-phytoestrogen diet prior to mating. Their female offspring were exposed to BPA, oocytes collected, and meiotic spindle and chromosome characteristics compared between control and BPA-treated females. We observed significant diet-related variation in both the frequency of abnormalities in oocytes from untreated females and in the response to BPA. Our results demonstrate that the impact of BPA on meiosis depends, at least in part, on diet. We suggest that variation in the conclusions of recent BPA studies reflects differences in the diets used, as well as other methodological differences. Because meiotic disturbances are a feature of all studies to date, however, we conclude that low levels of BPA adversely affect the meiotic process.

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Title: Bisphenol A effects on the growing mouse oocyte are influenced by diet
Creators: Ailene Muhlhauser - School of Molecular Biosciences, Washington State University, Pullman, Washington, USA
Martha Susiarjo
Carmen Rubio
Jodi Griswold
Galen Gorence
Terry Hassold
Patricia A Hunt
Publication Details: Biology of reproduction, Vol.80(5), pp.1066-1071
Academic Unit: Molecular Biosciences, School of
Publisher: United States
Grant note: R01 ES013527 / NIEHS NIH HHS ES013527 / NIEHS NIH HHS HD21341 / NICHD NIH HHS R01 HD021341 / NICHD NIH HHS R37 HD021341 / NICHD NIH HHS
Identifiers: 99900546618501842
Language: English
Resource Type: Journal article