Journal article
Bone cell–materials interaction on alumina ceramics with different grain sizes
Materials Science & Engineering C, Vol.29(4), pp.1201-1206
2009
Handle:
https://hdl.handle.net/2376/114420
Abstract
The objective of this work was to study adhesion, proliferation and differentiation of osteoblast cells (OPC1) on alumina ceramic, a bio-inert material. Alumina ceramic with different average grain sizes, 1 μm and 12 μm, respectively, were used in as-prepared condition without any grinding and polishing to understand the influence of grain size on cell–material interactions. Scanning electron microscopy and confocal imaging were used to study attachment, adhesion and differentiation of OPC1 cells. Cells attached, proliferated and differentiated well on both the substrates. Adhesion of cells, as assessed by observing the production of vinculin, was found to be a consistent phenomenon on both the substrates. On day 5 of cell culture, significant cell-attachment was observed and vinculin was detected throughout cytoplasm. MTT assay showed that proliferation of OPC1 cells was consistently higher in the case of 12 μm-alumina. Cells of different morphology, nodular, plate-like as well as elongated, were found to get anchored at grains, grain boundaries as well as pores. On day 16, there were clear signs of mineralization as well. Over all, alumina with average grain size of 12 μm showed better cell-attachment, growth and differentiation compared to 1 μm grain size samples.
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Details
- Title
- Bone cell–materials interaction on alumina ceramics with different grain sizes
- Creators
- Abhijit Chanda - Department of Mechanical Engineering, Jadavpur University, Kolkata 700032, IndiaRajdeep SinghaRoy - Central Glass & Ceramic Research Institute, Kolkata 700032, IndiaWeichung Xue - W. M. Keck Biomedical Materials Research Laboratory, School of Mechanical and Materials Engineering, Washington State University, Pullman, Washington 99164, USASusmita Bose - W. M. Keck Biomedical Materials Research Laboratory, School of Mechanical and Materials Engineering, Washington State University, Pullman, Washington 99164, USAAmit Bandyopadhyay - W. M. Keck Biomedical Materials Research Laboratory, School of Mechanical and Materials Engineering, Washington State University, Pullman, Washington 99164, USA
- Publication Details
- Materials Science & Engineering C, Vol.29(4), pp.1201-1206
- Academic Unit
- Mechanical and Materials Engineering, School of
- Publisher
- Elsevier B.V
- Identifiers
- 99900548312601842
- Language
- English
- Resource Type
- Journal article