Bordetella bronchiseptica responses to physiological reactive nitrogen and oxygen stresses

Anders Omsland; Katrina M Miranda; Richard L Friedman; Scott Boitano

doi:10.1111/j.1574-6968.2008.01181.x

Back

Bordetella bronchiseptica responses to physiological reactive nitrogen and oxygen stresses

Journal article

Open access

Peer reviewed

Bordetella bronchiseptica responses to physiological reactive nitrogen and oxygen stresses

Anders Omsland, Katrina M Miranda, Richard L Friedman and Scott Boitano

FEMS microbiology letters, Vol.284(1), pp.92-101

07/01/2008

DOI: https://doi.org/10.1111/j.1574-6968.2008.01181.x

Handle:

https://hdl.handle.net/2376/110409

PMCID: PMC2517120

PMID: 18462394

Files and links (1)

url

https://doi.org/10.1111/j.1574-6968.2008.01181.xView

Published (Version of record) Open

Abstract

bacterial stress

nitric oxide

reactive nitrogen species (RNS)

reactive oxygen species (ROS)

superoxide

hydrogen peroxide

Abstract Bordetella bronchiseptica can establish prolonged airway infection consistent with a highly developed ability to evade mammalian host immune responses. Upon initial interaction with the host upper respiratory tract mucosa, B. bronchiseptica are subjected to antimicrobial reactive nitrogen species (RNS) and reactive oxygen species (ROS), effector molecules of the innate immune system. However, the responses of B. bronchiseptica to redox species at physiologically relevant concentrations (nM–μM) have not been investigated. Using predicted physiological concentrations of nitric oxide (NO), superoxide and hydrogen peroxide (H2O2) on low numbers of CFU of B. bronchiseptica, all redox active species displayed dose-dependent antimicrobial activity. Susceptibility to individual redox active species was significantly increased upon introduction of a second species at subantimicrobial concentrations. An increased bacteriostatic activity of NO was observed relative to H2O2. The understanding of Bordetella responses to physiologically relevant levels of exogenous RNS and ROS will aid in defining the role of endogenous production of these molecules in host innate immunity against Bordetella and other respiratory pathogens.

Metrics

Details

Title: Bordetella bronchiseptica responses to physiological reactive nitrogen and oxygen stresses
Creators: Anders Omsland - Department of Cell Biology and Anatomy, Arizona Health Sciences Center, University of Arizona, AZ, USA
Katrina M Miranda - Department of Chemistry, University of Arizona, Tucson, AZ, USA
Richard L Friedman - Department of Immunobiology, Arizona Health Sciences Center, University of Arizona, AZ, USA
Scott Boitano - Department of Cell Biology and Anatomy, Arizona Health Sciences Center, University of Arizona, AZ, USA
Publication Details: FEMS microbiology letters, Vol.284(1), pp.92-101
Academic Unit: Paul G. Allen School for Global Animal Health
Publisher: Blackwell Publishing Ltd; Oxford, UK
Identifiers: 99900547119901842
Language: English
Resource Type: Journal article