Journal article
Bovine Leukemia Virus Transmembrane Protein gp30 Physically Associates with the Down-Regulatory Phosphatase SHP-1
Cellular immunology, Vol.193(2), pp.117-124
05/01/1999
Handle:
https://hdl.handle.net/2376/113258
PMID: 10222053
Abstract
In B lymphocytes, the down-regulatory phosphatase SHP-1 associates with CD22 and CD32b (also known as FcγRIIB) and acts as a critical negative regulator of B-cell receptor signaling. Bovine leukemia virus, a retrovirus of the HTLV/BLV group, causes persistently increased numbers of peripheral blood B lymphocytes, known as persistent lymphocytosis (PL) and, in some animals, progression to B-cell leukemia and/or lymphoma. Here, we show that SHP-1 associates with the bovine leukemia virus transmembrane protein, gp30. This interaction is either direct or indirect. The interaction is dependent on tyrosine phosphorylation, and the interaction increases after cell stimulation with sodium pervanadate. The gp30–SHP-1 interaction is seen in all of the BLV-infected, PL animals tested, but is not seen in uninfected animals or in most BLV-infected, non-PL animals, which do not express significant quantities of gp30. However, one BLV-infected, non-PL animal expressed large quantities of gp30, yet no gp30–SHP-1 interaction was detected, suggesting that there may be other factors in cells from the PL animals that facilitate the gp30–SHP-1 interaction. The association of gp30 and SHP-1 suggests the hypothesis that gp30 may act as a decoy to sequester SHP-1, resulting in up-regulation of B-cell receptor signaling. The implication of this could be a novel mechanism of viral activation of lymphocytes by removal of a down-regulatory phosphatase.
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Details
- Title
- Bovine Leukemia Virus Transmembrane Protein gp30 Physically Associates with the Down-Regulatory Phosphatase SHP-1
- Creators
- Glenn H Cantor - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, 99164-7040Suzanne M Pritchard - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, 99164-7040Oto Orlik - Department of Animal Health and Biomedical Sciences, University of Wisconsin, Madison, Wisconsin, 53706Gary A Splitter - Department of Animal Health and Biomedical Sciences, University of Wisconsin, Madison, Wisconsin, 53706William C Davis - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, 99164-7040Raymond Reeves - Department of Biochemistry/Biophysics, Washington State University, Pullman, Washington, 99164-7040
- Publication Details
- Cellular immunology, Vol.193(2), pp.117-124
- Academic Unit
- Veterinary Microbiology and Pathology, Department of; Molecular Biosciences, School of
- Publisher
- Elsevier Inc
- Identifiers
- 99900547609901842
- Language
- English
- Resource Type
- Journal article