Journal article
Bovine WC1(-) gammadeltaT cells incubated with IL-15 express the natural cytotoxicity receptor CD335 (NKp46) and produce IFN-gamma in response to exogenous IL-12 and IL-18
Developmental and comparative immunology, Vol.32(8), pp.1002-1010
2008
Handle:
https://hdl.handle.net/2376/116499
PMID: 18329100
Abstract
The gammadeltaT cells of ruminants are believed to participate in innate immunity and have been described with regulatory, inflammatory and cytotoxic functions. Here we describe a subset of CD3(+) TcR1(+) WC1(-)gammadeltaT cells expressing CD335 (NKp46), classically associated with CD3(-) natural killer (NK) cells, as a consequence of incubation with IL-15. This population, undetectable at the time of collection, developed after 2 week of IL-15 culture from splenic leukocytes (SPL) reaching greater than 50% of the total gammadeltaT cells. However, they did not grow well from peripheral blood leukocytes (PBL). Splenic gammadeltaT cells positively selected by magnetic separation prior to incubation with IL-15 and analyzed by flow cytometry, consistently yielded CD3(+) cells expressing CD335. These cells arose from the CD335(-)gammadeltaT cell population suggesting that the new population represents up-regulation of CD335 by gammadeltaT cells. CD335 mRNA expression from sorted IL-15-incubated SPL CD335(+)gammadeltaT cells or NK cells exceeded that of CD335(-)gammadeltaT cells. Incubation with IL-12 and IL-18 increased the number of CD335(+)gammadeltaT cells in both the PBL and SPL fractions as compared to controls or IL-12 or IL-18 alone. In addition, CD335(+)gammadeltaT cells demonstrated a robust ability to produce IFN-gamma in response to exogenous IL-12 and IL-18. Taken as a whole, we describe a new phenotypically distinct bovine gammadeltaT cell sub-population capable of participating in type 1 immune responses.
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Details
- Title
- Bovine WC1(-) gammadeltaT cells incubated with IL-15 express the natural cytotoxicity receptor CD335 (NKp46) and produce IFN-gamma in response to exogenous IL-12 and IL-18
- Creators
- Wendell C Johnson - Animal Disease Research Unit, USDA-ARS, Washington State University, Pullman, WA 99164-6630, USAReginaldo G BastosWilliam C DavisWill L Goff
- Publication Details
- Developmental and comparative immunology, Vol.32(8), pp.1002-1010
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- United States
- Identifiers
- 99900548003601842
- Language
- English
- Resource Type
- Journal article